|
| Status |
Public on Nov 30, 2016 |
| Title |
FAPs_pA_VMO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Fibro/adipogenic progenitors isolated from hindlimb skeletal muscles treated in vivo with pA-VMOs
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: skeletal muscle
age: 8-12 weeks
strain: C57/Bl6
treatment: Polyadenylation Site VMOs
|
| Treatment protocol |
Tibialis anterior muscles from C57Bl/6 mice were each injured with 30 µL of 50% v/v glycerol and injected with the indicated vivo morpholino (VMO) after 3 days. FAPs were then isolated from the muscles 2 days post-VMO injection.
|
| Growth protocol |
RNA was isolated from cells that were pelleted and snap-frozen immediately after isolation from the muscle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit as per the manufacturer’s instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated single stranded cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Fragmented biotin-labeled single stranded DNA was hybridized to Affymetrix Mouse Gene 1.0 Array according to the standard Affymetrix protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from WT fibro/adipogenic progenitors isolated from WT C57Bl/6 mice injured with glycerol, injected with pA-VMOs 3 days post-injury, and subsequently isolated 2 days post-VMO injection.
|
| Data processing |
The data were analyzed with the Affymertix Expression Console software using Affymetrix default RMA analysis settings.
|
| |
|
| Submission date |
May 23, 2016 |
| Last update date |
Nov 30, 2016 |
| Contact name |
Thomas A Rando |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University School of Medicine
|
| Department |
Neurology and Neurological Sciences
|
| Street address |
3801 Miranda Ave
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE81744 |
Intronic polyadenylation of PDGFRα in stromal stem cells attenuates muscle fibrosis |
|
