|
| Status |
Public on Dec 25, 2016 |
| Title |
PGC_dev5_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
PGC, stage: 5h20m - 7h20m AEL
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: primordial germ cells
developmental stage: 5h20m - 7h20m AEL
strain: EGFP-vasa
|
| Treatment protocol |
Embryo cells were dissociated by Tripsin treatment and then subjected to FACS sorting
|
| Growth protocol |
incubated at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy (QIAGEN) manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Protocol. 100ng of total RNA was used.
|
| |
|
| Channel 2 |
| Source name |
poole whole embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole embryos
developmental stage: 0 - 24h AEL
strain: EGFP-vasa
|
| Treatment protocol |
Embryo cells were dissociated by Tripsin treatment and then subjected to FACS sorting
|
| Growth protocol |
incubated at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy (QIAGEN) manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescent Linear Amplification Protocol. 100ng of total RNA was used.
|
| |
|
| |
| Hybridization protocol |
Agilent 60-mer oligo microarray processing protocol (SSC Wash/5-screw Hyb Chamber). See manufacturer's manual (#G4140-90010).
|
| Scan protocol |
Scanned using Agilent microarray scanner (G2565BA)
|
| Description |
PGCs (AEL:5h20m - 7h20m), biological replicate 2
|
| Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization
|
| |
|
| Submission date |
Jun 16, 2016 |
| Last update date |
Dec 25, 2016 |
| Contact name |
Shuji Shigenobu |
| E-mail(s) |
[email protected]
|
| Phone |
+81-564-55-7670
|
| Organization name |
National Institute for Basic Biology
|
| Lab |
Functional Genomics Facility
|
| Street address |
Nishigonaka 38, Myodaiji
|
| City |
Okazaki |
| State/province |
Aichi |
| ZIP/Postal code |
444-8585 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4336 |
| Series (1) |
|
