|
| Status |
Public on Jul 09, 2016 |
| Title |
5. Rb E2F4 |
| Sample type |
RNA |
| |
|
| Source name |
skin tissue, Rbf/f;K14creERTM;E2F4-/-, tamoxifen treated
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: FVB/N
genotype/variation: Rbf/f;K14creERTM;E2F4-/-
tissue: skin
|
| Treatment protocol |
Tamoxifen treatment was topically administered in the shaved backskin of animals (2x2 cm) at 20 mg/per day dissolved in DMSO/acetone for 5 consecutive days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with RNeasy Qiagen Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ngs total RNA (GeneChip Whole Transcript WT Expression Arrays, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.3 ug of DNAs were hybridized for 16 hr at 45C in the Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 7G.
|
| Data processing |
The data were analyzed with RMA using RMAExpress.
|
| |
|
| Submission date |
Jul 08, 2016 |
| Last update date |
Jul 09, 2016 |
| Contact name |
Monica Martinez-Fernandez |
| E-mail(s) |
[email protected]
|
| Organization name |
CIEMAT
|
| Street address |
Avda Complutense 40
|
| City |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE84206 |
Deregulation of the pRb-E2F4 axis alters epidermal homeostasis and favors tumor development |
|
