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Sample GSM22716 Query DataSets for GSM22716
Status Public on May 21, 2004
Title A chip: time 30 min Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 0.045940205 0 319.717 233.869 293.305 209.714 191 132 162 122
2 0.5753706 0 332.796 211.597 262.359 203.314 226 113 144 119.5
3 0.478734194 0 1631.72 233.208 952.454 239.929 1232 147 736 129
4 -0.119458399 0 306.104 260.916 313.379 276.704 199 160 184 164
5 -0.826489961 0 4588.87 256.625 5497.43 273.799 4039 148 4857 163
6 -0.216381506 0 740.468 226.148 739.519 242.48 528 122.5 528 143.5
7 -0.894476444 0 312.193 224.28 430.911 247.301 198 127 282 136
8 0.021688748 0 238.652 237.6 230.538 225.068 131.5 125.5 134 127.5
9 -0.416884348 0 2752.37 259.78 2566.69 282.285 2022 163 2026 166
10 0.091536435 0 246.288 235.339 254.834 236.122 138 124 154 147
11 -0.679124381 0 497.852 240.125 630.749 237.087 383 138 446 132
12 0.45275294 0 444.346 236.607 324.152 202.95 282.5 129.5 185.5 114
13 -0.994603866 0 2205.11 230.376 2934.21 253.094 2006 137 2706 153
14 -0.003919472 0 231.495 236.476 218.745 200.193 134.5 127 117 121.5
15 0.276274319 0 238.434 223.244 210.75 231.333 116 117 119 117
16 0.159098176 0 262.798 185.111 263.709 223.104 153.5 103.5 147.5 127.5
17 -0.285606023 0 465.474 198.544 515.475 231.003 323 105 325 126
18 -0.474211873 0 1262.03 232.094 1272.95 230.673 971 133 944 123
19 -0.133291225 0 217.282 225.75 210.366 230.364 105 130 113 119
20 -0.473989292 0 258.499 243.739 281.521 215.912 140 132 161 119

Total number of rows: 16928

Table truncated, full table size 1140 Kbytes.




Supplementary data files not provided

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