GEO help: Mouse over screen elements for information.
Status
Public on May 21, 2004
Title
A chip: time 30 min Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
0.045940205
0
319.717
233.869
293.305
209.714
191
132
162
122
2
0.5753706
0
332.796
211.597
262.359
203.314
226
113
144
119.5
3
0.478734194
0
1631.72
233.208
952.454
239.929
1232
147
736
129
4
-0.119458399
0
306.104
260.916
313.379
276.704
199
160
184
164
5
-0.826489961
0
4588.87
256.625
5497.43
273.799
4039
148
4857
163
6
-0.216381506
0
740.468
226.148
739.519
242.48
528
122.5
528
143.5
7
-0.894476444
0
312.193
224.28
430.911
247.301
198
127
282
136
8
0.021688748
0
238.652
237.6
230.538
225.068
131.5
125.5
134
127.5
9
-0.416884348
0
2752.37
259.78
2566.69
282.285
2022
163
2026
166
10
0.091536435
0
246.288
235.339
254.834
236.122
138
124
154
147
11
-0.679124381
0
497.852
240.125
630.749
237.087
383
138
446
132
12
0.45275294
0
444.346
236.607
324.152
202.95
282.5
129.5
185.5
114
13
-0.994603866
0
2205.11
230.376
2934.21
253.094
2006
137
2706
153
14
-0.003919472
0
231.495
236.476
218.745
200.193
134.5
127
117
121.5
15
0.276274319
0
238.434
223.244
210.75
231.333
116
117
119
117
16
0.159098176
0
262.798
185.111
263.709
223.104
153.5
103.5
147.5
127.5
17
-0.285606023
0
465.474
198.544
515.475
231.003
323
105
325
126
18
-0.474211873
0
1262.03
232.094
1272.95
230.673
971
133
944
123
19
-0.133291225
0
217.282
225.75
210.366
230.364
105
130
113
119
20
-0.473989292
0
258.499
243.739
281.521
215.912
140
132
161
119
Total number of rows: 16928 Table truncated, full table size 1140 Kbytes .Supplementary data files not provided