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Series GSE1393 Query DataSets for GSE1393
Status Public on May 21, 2004
Title A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treated with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Ref:Fang Y, Choi D, Searles RP, Mathers WD.A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.Invest Ophthalmol Vis Sci. 2005 Feb;46(2):461-9.
Keywords = Corneal injury
Keywords = mouse
Keywords = lacrimal gland
Keywords = gene microarrays
Keywords: time-course
 
 
Contributor(s) Fang Y, Choi D, Searles RP, Mathers WD
Citation(s) 15671270
Submission date May 11, 2004
Last update date Jan 17, 2013
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platforms (3)
GPL1230 SMCmou8400A
GPL1231 SMCmou6600Av1
GPL1232 SMCmou6600Av2
Samples (93)
GSM22716 A chip: time 30 min Replicate 1
GSM22717 A chip: time 30 min Replicate 2
GSM22718 A chip: time 30 min Replicate 3
Relations
BioProject PRJNA90175

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Supplementary data files not provided

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