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Status
Public on May 21, 2004
Title
A chip: time 30 min Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
2.12E-02
0
278.352
205.184
259.231
201.718
178
118
139
115
2
-1.45E-01
0
269.174
238.639
268.955
243.606
153.5
128
155.5
129.5
3
2.16E-01
0
1359.7
223.078
917.687
248.59
1040
118
666
159
4
2.17E-01
0
266.414
180.616
258.796
233.298
149.5
102
137
129
5
-7.53E-01
0
2237.72
252.151
2325.89
245.347
1974
129
1869
146
6
-4.33E-01
0
515.847
255.602
550.902
217.203
360
123
373
112
7
-3.20E-01
0
298.67
206.02
330.803
242.27
180
119
223.5
129.5
8
-2.58E-01
0
219.798
223.255
220.688
216.409
111
125
129
119
9
-7.30E-01
0
1531.77
248.19
1571.88
215.014
1167
135
1221
115
10
1.67E-01
0
251.015
227.8
232.823
235.568
144
145
123
146
11
-1.17E+00
0
395.954
227.819
603.599
208.478
244
125.5
445
116
12
6.19E-01
0
457.893
236.419
321.113
230.466
319
128
186
130
13
-1.11E+00
0
2341.11
237.204
3010.28
258.651
2120
133
2830
150
14
-5.40E-02
0
265.69
261.191
233.332
212.636
157
133
126
109
15
-1.64E-01
0
232.449
229.627
237.938
219.304
124
118.5
129.5
123
16
-4.23E-01
0
238.727
253.678
258.694
219.943
135
126
157
123
17
-3.65E-01
0
462.327
259.264
459.723
219.019
315
142.5
281.5
114
18
-8.31E-01
0
1446.24
230.664
1599.64
225.98
1053
114.5
1205
116
19
-5.44E-01
0
232.305
236.156
239.623
220.522
130
138.5
133
121
20
-2.36E-01
0
291.882
221.19
279.789
198.227
163
133
161
110
Total number of rows: 16928 Table truncated, full table size 1091 Kbytes .Supplementary data files not provided