NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22717 Query DataSets for GSM22717
Status Public on May 21, 2004
Title A chip: time 30 min Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 2.12E-02 0 278.352 205.184 259.231 201.718 178 118 139 115
2 -1.45E-01 0 269.174 238.639 268.955 243.606 153.5 128 155.5 129.5
3 2.16E-01 0 1359.7 223.078 917.687 248.59 1040 118 666 159
4 2.17E-01 0 266.414 180.616 258.796 233.298 149.5 102 137 129
5 -7.53E-01 0 2237.72 252.151 2325.89 245.347 1974 129 1869 146
6 -4.33E-01 0 515.847 255.602 550.902 217.203 360 123 373 112
7 -3.20E-01 0 298.67 206.02 330.803 242.27 180 119 223.5 129.5
8 -2.58E-01 0 219.798 223.255 220.688 216.409 111 125 129 119
9 -7.30E-01 0 1531.77 248.19 1571.88 215.014 1167 135 1221 115
10 1.67E-01 0 251.015 227.8 232.823 235.568 144 145 123 146
11 -1.17E+00 0 395.954 227.819 603.599 208.478 244 125.5 445 116
12 6.19E-01 0 457.893 236.419 321.113 230.466 319 128 186 130
13 -1.11E+00 0 2341.11 237.204 3010.28 258.651 2120 133 2830 150
14 -5.40E-02 0 265.69 261.191 233.332 212.636 157 133 126 109
15 -1.64E-01 0 232.449 229.627 237.938 219.304 124 118.5 129.5 123
16 -4.23E-01 0 238.727 253.678 258.694 219.943 135 126 157 123
17 -3.65E-01 0 462.327 259.264 459.723 219.019 315 142.5 281.5 114
18 -8.31E-01 0 1446.24 230.664 1599.64 225.98 1053 114.5 1205 116
19 -5.44E-01 0 232.305 236.156 239.623 220.522 130 138.5 133 121
20 -2.36E-01 0 291.882 221.19 279.789 198.227 163 133 161 110

Total number of rows: 16928

Table truncated, full table size 1091 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap