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Sample GSM22718 Query DataSets for GSM22718
Status Public on May 21, 2004
Title A chip: time 30 min Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 2.69E-02 0 291.443 244.27 252.264 194.319 161 138.5 144 105.5
2 -3.30E-01 0 262.532 248.52 237.404 214.691 149 147 130 104.5
3 4.38E-01 0 1238.16 258.326 880.909 213.742 992 137.5 677 122.5
4 -5.09E-01 0 224.874 248.98 216.366 200.424 120 137.5 114 104
5 -3.25E-01 0 892.953 238.201 972.337 206.439 721 135 759 116
6 1.15E-01 0 368.273 206.102 358.231 193.978 232 109 210 118
7 2.29E-01 0 246.002 184.603 244.769 228.444 138 99 128 126
8 3.20E-01 0 244.607 196.293 211.643 201.544 137 118 113 117
9 1.42E-01 0 628.964 240.431 589.752 233.307 472 125 390 129
10 -1.00E-01 0 240.268 221.605 227.426 202.812 140 117 131 105
11 -6.81E-01 0 246.392 228.379 286.911 204.064 139.5 128 161 112.5
12 3.24E-01 0 391.073 245.946 309.531 176.589 257 137 161 102
13 -4.21E-01 0 1411.29 235.045 1599.31 199.801 1211 126 1276 121
14 2.74E-01 0 218.202 217.591 212.683 223.828 117 123 120.5 137.5
15 1.12E+00 0 251.648 236.07 186.002 228.111 143 124.5 100 127.5
16 -1.37E-01 0 249.275 226.668 216.458 182.39 139 127 120 92
17 -9.40E-02 0 289.068 205.053 312.233 220.996 168 107 189.5 115
18 -4.96E-01 0 457.184 244.109 569.65 191.062 303 125 409 108
19 2.20E-01 0 218.254 203.598 210.742 223.553 119 113 108 126
20 1.02E-01 0 273.729 219.838 249.224 191.773 159 119.5 135 109

Total number of rows: 16928

Table truncated, full table size 1093 Kbytes.




Supplementary data files not provided

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