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Status
Public on May 21, 2004
Title
A chip: time 30 min Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
2.69E-02
0
291.443
244.27
252.264
194.319
161
138.5
144
105.5
2
-3.30E-01
0
262.532
248.52
237.404
214.691
149
147
130
104.5
3
4.38E-01
0
1238.16
258.326
880.909
213.742
992
137.5
677
122.5
4
-5.09E-01
0
224.874
248.98
216.366
200.424
120
137.5
114
104
5
-3.25E-01
0
892.953
238.201
972.337
206.439
721
135
759
116
6
1.15E-01
0
368.273
206.102
358.231
193.978
232
109
210
118
7
2.29E-01
0
246.002
184.603
244.769
228.444
138
99
128
126
8
3.20E-01
0
244.607
196.293
211.643
201.544
137
118
113
117
9
1.42E-01
0
628.964
240.431
589.752
233.307
472
125
390
129
10
-1.00E-01
0
240.268
221.605
227.426
202.812
140
117
131
105
11
-6.81E-01
0
246.392
228.379
286.911
204.064
139.5
128
161
112.5
12
3.24E-01
0
391.073
245.946
309.531
176.589
257
137
161
102
13
-4.21E-01
0
1411.29
235.045
1599.31
199.801
1211
126
1276
121
14
2.74E-01
0
218.202
217.591
212.683
223.828
117
123
120.5
137.5
15
1.12E+00
0
251.648
236.07
186.002
228.111
143
124.5
100
127.5
16
-1.37E-01
0
249.275
226.668
216.458
182.39
139
127
120
92
17
-9.40E-02
0
289.068
205.053
312.233
220.996
168
107
189.5
115
18
-4.96E-01
0
457.184
244.109
569.65
191.062
303
125
409
108
19
2.20E-01
0
218.254
203.598
210.742
223.553
119
113
108
126
20
1.02E-01
0
273.729
219.838
249.224
191.773
159
119.5
135
109
Total number of rows: 16928 Table truncated, full table size 1093 Kbytes .Supplementary data files not provided