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Status
Public on May 21, 2004
Title
A chip: time 30 min Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.74E-01
0
269.019
226.519
215.707
181.626
166.5
111
125
88
2
1.18E-01
0
268.584
219.19
227.334
187.248
153
115.5
115.5
99.5
3
3.58E-01
0
776.06
252.591
571.605
171.03
636
149.5
417
96
4
3.75E-01
0
260.216
211.716
225.011
225.376
164
105
117
122
5
-5.56E-01
0
1284.87
254.644
1583.75
240.159
1064
153
1313
117.5
6
-2.96E-02
0
356.224
222.776
337.914
195.612
228
115
193
105
7
1.61E-01
0
270.913
219.629
239.927
199.223
153
107
124
105
8
-3.80E-01
0
243.552
243.826
218.186
184.519
136
136
120
97.5
9
-4.17E-01
0
1025.48
252.534
1199.43
233.301
857
140
903
133
10
2.98E-01
0
262.368
247.22
200.566
184.698
151
121
111
102
11
-7.44E-01
0
360.96
286.97
420.845
181.312
224
157.5
276
96
12
4.38E-01
0
479.807
266.667
326.993
208.706
323
168
206
105
13
-6.21E-01
0
1292.31
261.837
1630.58
198.125
1007
165.5
1223
105.5
14
7.93E-01
0
274.035
225.33
195.273
206.299
170
119
104
117
15
9.36E-01
0
235.398
239.701
188.989
240.602
140.5
130.5
108.5
130
16
-4.47E-01
0
264.005
263.852
238.118
201.399
152
144.5
126
96
17
2.13E-01
0
373.223
217.577
317.209
206.428
234.5
125.5
175
116
18
-2.24E-02
0
805.186
209.401
787.854
206.556
618
113
585
123
19
4.48E-01
0
268.922
197.979
198.355
216.057
149
118.5
103
104
20
-3.84E-01
0
254.053
234.174
217.783
203.348
150
153.5
110
103.5
Total number of rows: 16928 Table truncated, full table size 1089 Kbytes .Supplementary data files not provided