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Sample GSM22722 Query DataSets for GSM22722
Status Public on May 21, 2004
Title A chip: time 1 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.72E-01 0 829.783 259.044 635.647 378.862 737 155 563 310
2 -4.78E-01 0 719.417 580.326 695.151 733.034 636 434 595 482
3 3.49E-01 0 9575.28 408.91 3080.91 426.427 10659 321 3103 354
4 -8.14E-01 0 457.505 396.418 369.572 267.787 358 292 291 195
5 -7.09E-02 0 15283 524.719 4849.88 379.111 16686 390 4906 296.5
6 NULL 0 1581 196.873 592.064 144.242 1488 136 434 75
7 -1.25E-01 0 894.619 146.629 553.683 156.485 777 77 450 73
8 NULL 0 176.417 176.107 131.211 228.832 103 68.5 67 138
9 -1.16E+00 0 3860.65 728.06 3724 840.481 3398 233 3335 265
10 -2.14E-01 0 265.412 150.453 222.024 136.484 169 81 168 86
11 -1.28E+00 0 1766.84 194.362 1968.99 179.876 1621 128.5 1883 91
12 1.48E+00 0 1075.15 169.181 302.441 170.05 477 100.5 193 99
13 -1.42E+00 0 6679.75 201.008 5533.45 186.957 5389 126 4395 116
14 2.22E-02 0 212.605 182.493 138.686 149.908 131 108 75 57.5
15 NULL 0 316.027 150.196 164.128 127.061 214 68 101 59.5
16 4.29E-02 0 431.374 152.94 232.015 120.474 336 86.5 160 48.5
17 2.03E-01 0 2053.52 173.564 976.851 158.366 1861 94.5 766 92
18 -7.94E-01 0 3938.12 193.576 3037.17 168.723 4035 135 2934 98.5
19 -3.41E-01 0 170.441 165.972 153.113 155.652 102 126 79 103
20 NULL 0 885.383 186.949 279.667 138.596 742 126 181 71

Total number of rows: 16928

Table truncated, full table size 1095 Kbytes.




Supplementary data files not provided

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