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Status
Public on May 21, 2004
Title
A chip: time 1 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.72E-01
0
829.783
259.044
635.647
378.862
737
155
563
310
2
-4.78E-01
0
719.417
580.326
695.151
733.034
636
434
595
482
3
3.49E-01
0
9575.28
408.91
3080.91
426.427
10659
321
3103
354
4
-8.14E-01
0
457.505
396.418
369.572
267.787
358
292
291
195
5
-7.09E-02
0
15283
524.719
4849.88
379.111
16686
390
4906
296.5
6
NULL
0
1581
196.873
592.064
144.242
1488
136
434
75
7
-1.25E-01
0
894.619
146.629
553.683
156.485
777
77
450
73
8
NULL
0
176.417
176.107
131.211
228.832
103
68.5
67
138
9
-1.16E+00
0
3860.65
728.06
3724
840.481
3398
233
3335
265
10
-2.14E-01
0
265.412
150.453
222.024
136.484
169
81
168
86
11
-1.28E+00
0
1766.84
194.362
1968.99
179.876
1621
128.5
1883
91
12
1.48E+00
0
1075.15
169.181
302.441
170.05
477
100.5
193
99
13
-1.42E+00
0
6679.75
201.008
5533.45
186.957
5389
126
4395
116
14
2.22E-02
0
212.605
182.493
138.686
149.908
131
108
75
57.5
15
NULL
0
316.027
150.196
164.128
127.061
214
68
101
59.5
16
4.29E-02
0
431.374
152.94
232.015
120.474
336
86.5
160
48.5
17
2.03E-01
0
2053.52
173.564
976.851
158.366
1861
94.5
766
92
18
-7.94E-01
0
3938.12
193.576
3037.17
168.723
4035
135
2934
98.5
19
-3.41E-01
0
170.441
165.972
153.113
155.652
102
126
79
103
20
NULL
0
885.383
186.949
279.667
138.596
742
126
181
71
Total number of rows: 16928 Table truncated, full table size 1095 Kbytes .Supplementary data files not provided