NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22723 Query DataSets for GSM22723
Status Public on May 21, 2004
Title A chip: time 1 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -6.54E-02 0 503.476 207.453 496.668 285.649 445 134.5 406.5 201
2 2.83E-01 0 607.129 469.123 542.849 536.446 546 359 461 387
3 1.47E-01 0 15068.3 403.195 5825.66 361.628 16020 348.5 5808 296.5
4 -1.28E+00 0 266.311 273.996 293.183 222.295 186.5 201.5 224 163
5 -1.17E+00 0 7063.34 266.657 6655.3 302.475 6346 206 5748 251
6 -2.21E-01 0 930.772 221.923 776.787 215.669 831 126.5 640 145.5
7 -3.50E-01 0 277.685 131.744 340.851 210.528 184 55.5 259 123
8 -9.66E-01 0 303.211 162.366 426.055 189.984 203 88.5 329 101
9 -1.44E+00 0 1804.69 147.631 2488.41 221.252 1575 62 2097 154
10 1.74E-02 0 174.782 120.92 246.634 228.391 97 48.5 172 146.5
11 -1.22E+00 0 282.41 134.251 527.102 192.58 231 52 476 115
12 NULL 0 125.593 149.529 233.539 213.481 47 63 170 133
13 -1.51E+00 0 3526.21 167.561 4462.93 250.876 3577 78 4568 190
14 NULL 0 134.246 142.071 210.657 195.073 53 67 153 112.5
15 -4.50E-02 0 191.625 152.573 206.617 204.343 101 72 128 107
16 -3.56E-01 0 218.766 150.5 293.644 229.907 140.5 81 229 158
17 -1.02E-01 0 999.94 160.538 798.195 208.222 822.5 74.5 655 147
18 -1.27E+00 0 1765.06 159.294 2235.59 240.532 1505 73 1811 182.5
19 -1.74E-01 0 162.724 166.141 237.31 238.351 77.5 72.5 163.5 153
20 -2.48E-01 0 332.235 163.029 362.982 219.408 224 92 290 143

Total number of rows: 16928

Table truncated, full table size 1090 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap