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Status
Public on May 21, 2004
Title
A chip: time 1 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-6.54E-02
0
503.476
207.453
496.668
285.649
445
134.5
406.5
201
2
2.83E-01
0
607.129
469.123
542.849
536.446
546
359
461
387
3
1.47E-01
0
15068.3
403.195
5825.66
361.628
16020
348.5
5808
296.5
4
-1.28E+00
0
266.311
273.996
293.183
222.295
186.5
201.5
224
163
5
-1.17E+00
0
7063.34
266.657
6655.3
302.475
6346
206
5748
251
6
-2.21E-01
0
930.772
221.923
776.787
215.669
831
126.5
640
145.5
7
-3.50E-01
0
277.685
131.744
340.851
210.528
184
55.5
259
123
8
-9.66E-01
0
303.211
162.366
426.055
189.984
203
88.5
329
101
9
-1.44E+00
0
1804.69
147.631
2488.41
221.252
1575
62
2097
154
10
1.74E-02
0
174.782
120.92
246.634
228.391
97
48.5
172
146.5
11
-1.22E+00
0
282.41
134.251
527.102
192.58
231
52
476
115
12
NULL
0
125.593
149.529
233.539
213.481
47
63
170
133
13
-1.51E+00
0
3526.21
167.561
4462.93
250.876
3577
78
4568
190
14
NULL
0
134.246
142.071
210.657
195.073
53
67
153
112.5
15
-4.50E-02
0
191.625
152.573
206.617
204.343
101
72
128
107
16
-3.56E-01
0
218.766
150.5
293.644
229.907
140.5
81
229
158
17
-1.02E-01
0
999.94
160.538
798.195
208.222
822.5
74.5
655
147
18
-1.27E+00
0
1765.06
159.294
2235.59
240.532
1505
73
1811
182.5
19
-1.74E-01
0
162.724
166.141
237.31
238.351
77.5
72.5
163.5
153
20
-2.48E-01
0
332.235
163.029
362.982
219.408
224
92
290
143
Total number of rows: 16928 Table truncated, full table size 1090 Kbytes .Supplementary data files not provided