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Status
Public on May 21, 2004
Title
A chip: time 1 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-3.64E-01
0
301.119
266.189
261.976
194.118
163.5
151
147
105.5
2
-1.43E-01
0
324.752
259.833
280.605
243.331
195
144
166
114
3
8.12E-01
0
3692.09
251.026
1642.35
220.811
3505
134.5
1407
113.5
4
-1.86E-01
0
277.989
216.376
233.791
182.533
154
131
120
98
5
-3.03E-01
0
4831.23
266.48
3742.51
241.523
4031
169.5
3323
148
6
6.29E-01
0
661.969
256.982
453.505
215.11
511
145
328
115
7
-5.67E-02
0
334.226
228.324
291.148
169.508
207
120.5
159
99
8
-5.43E-01
0
236.598
250.093
198.739
172.993
118
139
101
85
9
-4.46E-01
0
1361.13
212.211
1451.81
189.767
1037
109.5
994
94.5
10
-2.70E-01
0
218.259
204.27
202.748
203.626
108
107
106
95
11
-5.98E-01
0
330.579
189.771
422.818
178.19
206
93
264
98
12
1.05E+00
0
745.481
216.466
402.475
163.982
585
125
275
91
13
-1.27E+00
0
1100.15
187.909
1910.6
188.975
902
106
1548
100
14
6.64E-02
0
197.048
178.062
178.729
172.459
111
92.5
96
92
15
-8.34E-03
0
250.674
207.943
210.682
170.287
137
118
110
102
16
1.22E-02
0
211.526
197.094
193.94
193.047
116
95
98.5
98.5
17
-1.28E-01
0
431.887
191.473
432.594
172.126
307
102.5
268
91.5
18
-4.36E-01
0
929.437
203.752
1078.91
203.207
782
101
852
104
19
-3.70E-01
0
184.703
190.417
187.991
189.792
93
107
101
97
20
-6.64E-02
0
313.558
187.282
284.768
129.134
181
95.5
151
86
Total number of rows: 16928 Table truncated, full table size 1089 Kbytes .Supplementary data files not provided