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Sample GSM22736 Query DataSets for GSM22736
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 6.37E-02 0 477.905 277.456 434.184 298.084 379 156 287 183
2 8.96E-01 0 662.413 387.531 528.658 453.861 531 274 423 349
3 7.57E-02 0 6382.41 340.698 3854.43 336.453 6146 226.5 3437 223
4 -6.19E-01 0 367.666 291.976 370.436 279.718 265 194.5 270 157.5
5 3.40E-01 0 11185.8 349.43 5625.09 370.017 10141 266 4761 252.5
6 2.38E-02 0 858.643 265.771 764.521 341.237 630 151 593 234
7 -2.92E-01 0 468.472 239.482 453.047 249.941 356 152 338 145
8 5.29E-01 0 274.149 250.633 280.2 304.651 154 150 169 196
9 -1.28E+00 0 2667.98 319.701 3726.91 295.061 1994 194.5 2691 187
10 8.07E-02 0 325.92 252.262 302.88 244.272 185 138.5 190 151.5
11 -1.14E+00 0 551.106 263.723 754.195 229.977 420 160.5 602 133
12 3.57E-01 0 2608.81 257.378 1487.57 294.41 2019 139 1103 157
13 -1.60E+00 0 2456.99 241.848 4275.62 324.346 2227 131 3918 218
14 -2.78E-01 0 251.478 277.116 262.467 264.461 142 156.5 150 156
15 -9.33E-01 0 273.777 258.172 289.645 221.649 167 171 180 122
16 3.09E-01 0 386.378 244.179 323.949 290.045 283 152 213 163
17 -5.21E-02 0 985.546 263.83 795.35 260.353 755 162 639 145
18 -7.80E-01 0 1974.22 264.128 2142.66 271.265 1592 169 1560 167.5
19 2.64E-01 0 275.76 223.208 246.009 221.235 156 114.5 136 124.5
20 4.71E-01 0 479.257 242.593 351.545 252.722 322 141.5 205 147.5

Total number of rows: 16928

Table truncated, full table size 1097 Kbytes.




Supplementary data files not provided

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