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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
6.37E-02
0
477.905
277.456
434.184
298.084
379
156
287
183
2
8.96E-01
0
662.413
387.531
528.658
453.861
531
274
423
349
3
7.57E-02
0
6382.41
340.698
3854.43
336.453
6146
226.5
3437
223
4
-6.19E-01
0
367.666
291.976
370.436
279.718
265
194.5
270
157.5
5
3.40E-01
0
11185.8
349.43
5625.09
370.017
10141
266
4761
252.5
6
2.38E-02
0
858.643
265.771
764.521
341.237
630
151
593
234
7
-2.92E-01
0
468.472
239.482
453.047
249.941
356
152
338
145
8
5.29E-01
0
274.149
250.633
280.2
304.651
154
150
169
196
9
-1.28E+00
0
2667.98
319.701
3726.91
295.061
1994
194.5
2691
187
10
8.07E-02
0
325.92
252.262
302.88
244.272
185
138.5
190
151.5
11
-1.14E+00
0
551.106
263.723
754.195
229.977
420
160.5
602
133
12
3.57E-01
0
2608.81
257.378
1487.57
294.41
2019
139
1103
157
13
-1.60E+00
0
2456.99
241.848
4275.62
324.346
2227
131
3918
218
14
-2.78E-01
0
251.478
277.116
262.467
264.461
142
156.5
150
156
15
-9.33E-01
0
273.777
258.172
289.645
221.649
167
171
180
122
16
3.09E-01
0
386.378
244.179
323.949
290.045
283
152
213
163
17
-5.21E-02
0
985.546
263.83
795.35
260.353
755
162
639
145
18
-7.80E-01
0
1974.22
264.128
2142.66
271.265
1592
169
1560
167.5
19
2.64E-01
0
275.76
223.208
246.009
221.235
156
114.5
136
124.5
20
4.71E-01
0
479.257
242.593
351.545
252.722
322
141.5
205
147.5
Total number of rows: 16928 Table truncated, full table size 1097 Kbytes .Supplementary data files not provided