GEO help: Mouse over screen elements for information.
Status
Public on May 21, 2004
Title
A chip: time 24 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-2.32E-01
0
276.694
248.434
248.062
231.155
206
158
171
126
2
NULL
0
493.762
409.361
479.437
463.258
447
330
414
342
3
7.18E-01
0
1590.74
240.087
723.765
292.408
1270
153
593
212
4
1.48E-01
0
199.269
162.063
207.908
208.211
137
85
131
117
5
-9.21E-02
0
1355.14
203.014
846.26
213.413
1163
130
723
135
6
-7.07E-02
0
269.166
179.757
238.131
172.686
185
94
168
99
7
-2.94E-01
0
228.843
187.511
194.497
146.932
145
91
102
59
8
4.74E-01
0
191.617
167.906
149.468
151.536
107
76
69
73
9
-1.69E-01
0
685.381
210.549
476.778
167.415
567
130
379
85
10
-8.25E-01
0
190.916
190.721
164.413
137.721
107
118.5
73
55
11
-6.50E-01
0
208.676
159.209
210.714
146.566
142
96.5
138
65
12
6.08E-01
0
689.04
167.261
362.75
152.417
507
92.5
247
96
13
-3.69E-01
0
633.721
185.3
456.413
131.319
506
127
364
49
14
-1.76E-01
0
185.375
172.149
156.413
152
115
94
75.5
65
15
-6.61E-01
0
188.118
207.456
154.284
159.592
105
135
78
78
16
-2.19E-01
0
186.601
220.323
159.553
162.177
103
116.5
79
87
17
-3.31E-01
0
269.073
184.708
200.847
112.461
173
74
112
22
18
-1.06E-01
0
433.339
178.869
298.805
142.59
353
114.5
221
55.5
19
2.24E-01
0
170.931
161.091
146.869
165.201
87
84
71
80
20
1.96E-01
0
224.216
174.086
171.012
153.4
159.5
87
99.5
63.5
Total number of rows: 16928 Table truncated, full table size 1086 Kbytes .Supplementary data files not provided