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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
4.66E-01
0
143.22
84.8165
122.687
75.2706
59
15.5
47
12
2
-1.87E-02
0
97.4594
73.5315
108.741
90.1772
20
16
30
27.5
3
1.70E+00
0
695.388
72.6958
349.095
81.9353
496
15
243
13
4
8.39E-02
0
109.031
71.0839
115.468
76.0963
27
15
30
16
5
3.33E-01
0
1072.46
86.4796
1422.39
110.548
865
21.5
1153
28
6
2.53E-01
0
123.049
73.7886
122.439
63.7752
35
10
39
10
7
-6.56E-01
0
82.7122
88.2926
104.243
73.1915
13
22.5
31
9
8
-8.49E-01
0
54.5856
68.9141
72.0788
67.9293
8
13
11
9.5
9
-2.50E-01
0
322.701
59.1131
644.153
67.2353
224
7
502
10
10
2.04E-01
0
85.4127
55.8483
73.161
58.0069
21.5
9
15
9
11
3.73E-01
0
133.369
74.4934
124.652
89.4672
42
9
36
9
12
3.91E-01
0
106.226
79.3836
76.3048
61.2862
22
15
11
9
13
5.75E-01
0
98.8379
64.3012
66.275
68.9472
19
10
9
9
14
-1.40E-01
0
63.1584
40.873
68.9149
74.5159
10
5
9
11
15
NULL
0
68.0546
61.5136
90.6648
89.4388
10
9.5
26
9
16
1.34E-01
0
94.0419
60.6779
85.8087
65.7114
20
8
19
9
17
NULL
0
104.958
71.7608
88.6503
78.9412
23
12
15
11
18
-8.70E-02
0
267.985
70.7087
444.219
58.2427
176
10
337
9
19
-7.92E-03
0
68.9089
62.371
58.3843
53.4932
13
11
8
9
20
1.91E-01
0
87.4117
65.1812
75.9581
67.9396
16
10
12
10
Total number of rows: 16928 Table truncated, full table size 1000 Kbytes .Supplementary data files not provided