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Sample GSM22751 Query DataSets for GSM22751
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 4.66E-01 0 143.22 84.8165 122.687 75.2706 59 15.5 47 12
2 -1.87E-02 0 97.4594 73.5315 108.741 90.1772 20 16 30 27.5
3 1.70E+00 0 695.388 72.6958 349.095 81.9353 496 15 243 13
4 8.39E-02 0 109.031 71.0839 115.468 76.0963 27 15 30 16
5 3.33E-01 0 1072.46 86.4796 1422.39 110.548 865 21.5 1153 28
6 2.53E-01 0 123.049 73.7886 122.439 63.7752 35 10 39 10
7 -6.56E-01 0 82.7122 88.2926 104.243 73.1915 13 22.5 31 9
8 -8.49E-01 0 54.5856 68.9141 72.0788 67.9293 8 13 11 9.5
9 -2.50E-01 0 322.701 59.1131 644.153 67.2353 224 7 502 10
10 2.04E-01 0 85.4127 55.8483 73.161 58.0069 21.5 9 15 9
11 3.73E-01 0 133.369 74.4934 124.652 89.4672 42 9 36 9
12 3.91E-01 0 106.226 79.3836 76.3048 61.2862 22 15 11 9
13 5.75E-01 0 98.8379 64.3012 66.275 68.9472 19 10 9 9
14 -1.40E-01 0 63.1584 40.873 68.9149 74.5159 10 5 9 11
15 NULL 0 68.0546 61.5136 90.6648 89.4388 10 9.5 26 9
16 1.34E-01 0 94.0419 60.6779 85.8087 65.7114 20 8 19 9
17 NULL 0 104.958 71.7608 88.6503 78.9412 23 12 15 11
18 -8.70E-02 0 267.985 70.7087 444.219 58.2427 176 10 337 9
19 -7.92E-03 0 68.9089 62.371 58.3843 53.4932 13 11 8 9
20 1.91E-01 0 87.4117 65.1812 75.9581 67.9396 16 10 12 10

Total number of rows: 16928

Table truncated, full table size 1000 Kbytes.




Supplementary data files not provided

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