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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal Mean
Cy5_Background Mean
Cy3_Signal Mean
Cy3_Background Mean
Cy5_Signal Median
Cy5_Background Median
Cy3_Signal Median
Cy3_Background Median
Data table
ID_REF
VALUE
Flag
Cy5_Signal Mean
Cy5_Background Mean
Cy3_Signal Mean
Cy3_Background Mean
Cy5_Signal Median
Cy5_Background Median
Cy3_Signal Median
Cy3_Background Median
1
1.18E-01
0
307.3104895
242.1866029
216.8391608
174.5789474
249
166
134
95
2
-1.71E-01
0
277.3314685
258.9160839
316.1748252
312.7482517
214
171
261
202.5
3
-8.06E-02
0
498.8415301
239.3823529
439.2422587
236.0698529
425
179
328
143.5
4
-9.68E-03
0
278.545045
250.9509202
189.1291291
167.7208589
216
178
101.5
91.5
5
2.00E-01
0
1098.243243
270.5798611
715.5278219
140.2569444
951
182.5
577
66.5
6
6.17E-01
0
339.5993641
202.305
203.0508744
167.28
284
130
110
85
7
7.03E-01
0
275.0910747
234.1672355
147.6557377
145.7576792
211
171
77
80
8
-6.27E-01
0
255.3813814
277.7735043
170.3033033
135.3846154
167
208.5
88
77
9
-1.24E-02
0
596.9672775
243.7526316
445.4057592
159.8157895
510.5
165.5
343
73
10
-2.60E-01
0
279.9034608
244.5303867
192.5956284
143.7127072
211
163.5
103
58.5
11
-5.79E-01
0
301.6806527
264.1133333
191.6713287
145.18
229
224.5
113.5
75.5
12
-2.55E-01
0
335.7363014
268.6833333
232.6386986
143.775
271.5
185.5
139
73.5
13
-2.90E-01
0
666.0418944
258.4920319
574.0255009
153.249004
586
181
457
67.5
14
4.27E-01
0
288.3952641
261.4877301
146.8233151
151.8067485
198
201
67
75.5
15
-7.67E-01
0
263.372028
275.8757062
165.0503497
121.0141243
195
205.5
83
54.5
16
NULL
0
315.7522769
297.1778656
144.6684882
172.5928854
280
226
61
81
17
1.24E-01
0
409.867031
283.3888889
212.8251366
145.0634921
369
218.5
136
61
18
-2.29E-01
0
537.7477477
246.9542484
431.4984985
136.127451
463
177.5
319
66
19
2.31E-01
0
319.4515103
239.5877437
176.0508744
136.2033426
266
168
95
57
20
-9.25E-02
0
299.2448331
275.0788732
164.354531
148.2
238
202
75
64
Total number of rows: 16928 Table truncated, full table size 1342 Kbytes .Supplementary data files not provided