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Sample GSM22755 Query DataSets for GSM22755
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal Mean
Cy5_Background Mean
Cy3_Signal Mean
Cy3_Background Mean
Cy5_Signal Median
Cy5_Background Median
Cy3_Signal Median
Cy3_Background Median

Data table
ID_REF VALUE Flag Cy5_Signal Mean Cy5_Background Mean Cy3_Signal Mean Cy3_Background Mean Cy5_Signal Median Cy5_Background Median Cy3_Signal Median Cy3_Background Median
1 1.18E-01 0 307.3104895 242.1866029 216.8391608 174.5789474 249 166 134 95
2 -1.71E-01 0 277.3314685 258.9160839 316.1748252 312.7482517 214 171 261 202.5
3 -8.06E-02 0 498.8415301 239.3823529 439.2422587 236.0698529 425 179 328 143.5
4 -9.68E-03 0 278.545045 250.9509202 189.1291291 167.7208589 216 178 101.5 91.5
5 2.00E-01 0 1098.243243 270.5798611 715.5278219 140.2569444 951 182.5 577 66.5
6 6.17E-01 0 339.5993641 202.305 203.0508744 167.28 284 130 110 85
7 7.03E-01 0 275.0910747 234.1672355 147.6557377 145.7576792 211 171 77 80
8 -6.27E-01 0 255.3813814 277.7735043 170.3033033 135.3846154 167 208.5 88 77
9 -1.24E-02 0 596.9672775 243.7526316 445.4057592 159.8157895 510.5 165.5 343 73
10 -2.60E-01 0 279.9034608 244.5303867 192.5956284 143.7127072 211 163.5 103 58.5
11 -5.79E-01 0 301.6806527 264.1133333 191.6713287 145.18 229 224.5 113.5 75.5
12 -2.55E-01 0 335.7363014 268.6833333 232.6386986 143.775 271.5 185.5 139 73.5
13 -2.90E-01 0 666.0418944 258.4920319 574.0255009 153.249004 586 181 457 67.5
14 4.27E-01 0 288.3952641 261.4877301 146.8233151 151.8067485 198 201 67 75.5
15 -7.67E-01 0 263.372028 275.8757062 165.0503497 121.0141243 195 205.5 83 54.5
16 NULL 0 315.7522769 297.1778656 144.6684882 172.5928854 280 226 61 81
17 1.24E-01 0 409.867031 283.3888889 212.8251366 145.0634921 369 218.5 136 61
18 -2.29E-01 0 537.7477477 246.9542484 431.4984985 136.127451 463 177.5 319 66
19 2.31E-01 0 319.4515103 239.5877437 176.0508744 136.2033426 266 168 95 57
20 -9.25E-02 0 299.2448331 275.0788732 164.354531 148.2 238 202 75 64

Total number of rows: 16928

Table truncated, full table size 1342 Kbytes.




Supplementary data files not provided

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