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Status
Public on May 21, 2004
Title
A chip: time 15 day Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.86E-02
0
377.945
188.419
291.856
188.63
292
120.5
217
113
2
-7.22E-01
0
222.889
186.703
409.863
431.339
146
123
338
274
3
3.49E-01
0
1613.37
163.098
1000.93
314.067
1438
88
899
274
4
-9.47E-01
0
194.438
167.307
184.81
113.729
111.5
94
106
29
5
-4.32E-01
0
5630.74
1576.71
3881.28
1225.55
5571
558.5
3835
333.5
6
-4.83E-01
0
530.293
212.658
438.672
165.772
376
142.5
339
65.5
7
-2.82E-01
0
256.986
145.694
217.437
132.62
172.5
70
126
51.5
8
9.80E-01
0
170.832
129.818
126.417
133.521
87
47.5
46
70
9
-8.81E-01
0
2434.66
214.503
2316.67
143.377
1956
105
1815
64
10
-2.29E-01
0
208.359
153.822
188.179
130.679
124
76
112
64
11
-7.40E-01
0
410.479
126.727
483.571
146.823
329
46.5
339
65.5
12
1.95E-01
0
479.02
133.85
318.49
155.526
376
54
244
63
13
-7.37E-01
0
3165.8
156.969
2694.9
122.051
3161
71
2473
52.5
14
-1.32E-01
0
132.622
125.5
122.214
110.252
49
43.5
42
36
15
-2.89E-01
0
140.73
121.706
138.293
115.508
57
48.5
55.5
39
16
-2.09E-01
0
162.545
140.573
158.469
136.013
78
59
76
53
17
3.38E-02
0
912.241
165.371
601.034
121.008
778.5
69
514
53
18
-7.42E-01
0
2110.36
150.615
1957.71
187.074
2019
74
1787
117
19
-4.94E-01
0
215.363
195.048
196.262
142.382
115.5
114
92
74.5
20
2.04E-01
0
225.666
134.473
174.357
129.105
133
46
89
54
Total number of rows: 16928 Table truncated, full table size 1058 Kbytes .Supplementary data files not provided