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Sample GSM22761 Query DataSets for GSM22761
Status Public on May 21, 2004
Title A chip: time 15 day Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 7.72E-02 0 252.322 152.63 270.05 197.637 188 79.5 189 132
2 -2.04E+00 0 238.399 244.675 451.405 388.771 158.5 187 372 265.5
3 1.04E-02 0 1219.26 198.421 873.355 210.462 1060 127 735 131
4 -6.73E-01 0 188.003 183.343 189.018 168.011 107.5 115.5 103 88
5 -4.77E-01 0 4230.03 261.898 3554.4 233.713 4017 171 3238 161.5
6 1.49E-01 0 465.168 179.266 360.158 175.807 371 76 236 87
7 2.11E-01 0 251.211 139.972 201.552 134.097 177 64.5 111 67
8 NULL 0 158.648 180.451 167.94 151.37 77 98 95 72
9 -7.54E-01 0 1751.53 195.559 1808.01 183.135 1621.5 129.5 1589 111
10 -5.78E-03 0 179.976 152.026 174.631 156.696 98 79.5 99 83
11 -4.57E-01 0 353.15 153.899 400.276 164.605 261 66.5 318 94
12 -6.82E-02 0 333.909 155.126 290.589 150.854 235 68.5 211 68.5
13 -6.96E-01 0 2081.99 169.866 2017.42 133.783 1994 97 1855 63
14 1.98E-01 0 161.741 152.434 160.373 146.081 89 64 86 85
15 -5.60E-02 0 163.986 153.425 154.127 161.95 89 86 73 79
16 -1.38E-02 0 181.574 161.965 167.682 151.342 112 72 80 69
17 1.68E-01 0 526.596 141.964 425.098 195.532 437 68.5 310.5 112
18 -7.70E-01 0 1216.56 167.518 1316.52 155.392 1044 95 1051 71
19 -3.23E-01 0 226.469 170.386 197.971 127.016 137.5 84.5 123 45
20 NULL 0 163.437 155.949 196.209 169.068 79 79 133 83

Total number of rows: 16928

Table truncated, full table size 1063 Kbytes.




Supplementary data files not provided

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