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Status
Public on May 21, 2004
Title
A chip: time 15 day Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
7.72E-02
0
252.322
152.63
270.05
197.637
188
79.5
189
132
2
-2.04E+00
0
238.399
244.675
451.405
388.771
158.5
187
372
265.5
3
1.04E-02
0
1219.26
198.421
873.355
210.462
1060
127
735
131
4
-6.73E-01
0
188.003
183.343
189.018
168.011
107.5
115.5
103
88
5
-4.77E-01
0
4230.03
261.898
3554.4
233.713
4017
171
3238
161.5
6
1.49E-01
0
465.168
179.266
360.158
175.807
371
76
236
87
7
2.11E-01
0
251.211
139.972
201.552
134.097
177
64.5
111
67
8
NULL
0
158.648
180.451
167.94
151.37
77
98
95
72
9
-7.54E-01
0
1751.53
195.559
1808.01
183.135
1621.5
129.5
1589
111
10
-5.78E-03
0
179.976
152.026
174.631
156.696
98
79.5
99
83
11
-4.57E-01
0
353.15
153.899
400.276
164.605
261
66.5
318
94
12
-6.82E-02
0
333.909
155.126
290.589
150.854
235
68.5
211
68.5
13
-6.96E-01
0
2081.99
169.866
2017.42
133.783
1994
97
1855
63
14
1.98E-01
0
161.741
152.434
160.373
146.081
89
64
86
85
15
-5.60E-02
0
163.986
153.425
154.127
161.95
89
86
73
79
16
-1.38E-02
0
181.574
161.965
167.682
151.342
112
72
80
69
17
1.68E-01
0
526.596
141.964
425.098
195.532
437
68.5
310.5
112
18
-7.70E-01
0
1216.56
167.518
1316.52
155.392
1044
95
1051
71
19
-3.23E-01
0
226.469
170.386
197.971
127.016
137.5
84.5
123
45
20
NULL
0
163.437
155.949
196.209
169.068
79
79
133
83
Total number of rows: 16928 Table truncated, full table size 1063 Kbytes .Supplementary data files not provided