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Sample GSM22763 Query DataSets for GSM22763
Status Public on May 21, 2004
Title B chip Version1: time 30 min Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 196.65 170.502 162.308 132.871 110 100 79 61
2 2.67E-01 0 235.234 199.091 200.409 185.745 165 102 110 88.5
3 -7.57E-02 0 1130.26 192.196 1201.87 215.226 925 120.5 879 139
4 -7.44E-01 0 210.843 205.08 177.036 149.78 130 157 103 82
5 2.55E-01 0 865.854 215.733 712.384 165.733 710 151 568 109
6 -1.34E-01 0 388.743 197.788 397.987 146.127 331 123 303 71
7 4.35E-01 0 212.124 160.97 193.45 182.837 132 101 106 106
8 3.62E-01 0 207.375 168.418 206.913 173.789 119 74 120 102
9 1.14E-01 0 233.444 141.113 286.359 182.052 155 53 186 119
10 -3.46E-01 0 180.501 158.111 195.817 145.746 90 79 102 63.5
11 NULL 0 179.408 157.032 215.461 168.14 97.5 73 131 101.5
12 -3.81E-01 0 182.572 159.292 217.326 168.76 122 87 127 88
13 5.74E-01 0 209.701 148.509 230.77 210.742 140 62 159 131
14 -1.09E+00 0 186.885 193.718 219.117 140.729 129 119 148 63
15 NULL 0 225.386 185.319 411.301 180.459 141 106.5 316 117.5
16 -1.75E-01 0 201.466 177.854 233.003 194.303 132 103.5 159 117
17 NULL 0 273.134 174.459 394.469 166.77 200 80 315 97.5
18 -6.82E-02 0 203.279 165.423 203.592 190.455 129.5 107 131 96
19 4.84E-01 0 215.959 171.621 203.259 175.126 147 100 127 115.5
20 -4.96E-02 0 219.827 208.597 180.952 170.983 132 145 94.5 98

Total number of rows: 14720

Table truncated, full table size 941 Kbytes.




Supplementary data files not provided

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