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Status
Public on May 21, 2004
Title
B chip Version1: time 30 min Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
196.65
170.502
162.308
132.871
110
100
79
61
2
2.67E-01
0
235.234
199.091
200.409
185.745
165
102
110
88.5
3
-7.57E-02
0
1130.26
192.196
1201.87
215.226
925
120.5
879
139
4
-7.44E-01
0
210.843
205.08
177.036
149.78
130
157
103
82
5
2.55E-01
0
865.854
215.733
712.384
165.733
710
151
568
109
6
-1.34E-01
0
388.743
197.788
397.987
146.127
331
123
303
71
7
4.35E-01
0
212.124
160.97
193.45
182.837
132
101
106
106
8
3.62E-01
0
207.375
168.418
206.913
173.789
119
74
120
102
9
1.14E-01
0
233.444
141.113
286.359
182.052
155
53
186
119
10
-3.46E-01
0
180.501
158.111
195.817
145.746
90
79
102
63.5
11
NULL
0
179.408
157.032
215.461
168.14
97.5
73
131
101.5
12
-3.81E-01
0
182.572
159.292
217.326
168.76
122
87
127
88
13
5.74E-01
0
209.701
148.509
230.77
210.742
140
62
159
131
14
-1.09E+00
0
186.885
193.718
219.117
140.729
129
119
148
63
15
NULL
0
225.386
185.319
411.301
180.459
141
106.5
316
117.5
16
-1.75E-01
0
201.466
177.854
233.003
194.303
132
103.5
159
117
17
NULL
0
273.134
174.459
394.469
166.77
200
80
315
97.5
18
-6.82E-02
0
203.279
165.423
203.592
190.455
129.5
107
131
96
19
4.84E-01
0
215.959
171.621
203.259
175.126
147
100
127
115.5
20
-4.96E-02
0
219.827
208.597
180.952
170.983
132
145
94.5
98
Total number of rows: 14720 Table truncated, full table size 941 Kbytes .Supplementary data files not provided