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Sample GSM22770 Query DataSets for GSM22770
Status Public on May 21, 2004
Title B chip Version1: time 1 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 185.046 180.007 153.313 164.414 103 97.5 75.5 91.5
2 1.99E-01 0 216.719 171.246 172.146 165.101 141 105.5 79 75.5
3 9.28E-01 0 2344.32 204.221 1270.92 185.111 2072 127 957 118
4 -4.12E-01 0 190.63 190.208 173.007 164.531 124 122 98 77.5
5 8.17E-01 0 2330.58 179.068 1307.47 141.963 2097 112.5 1113 70.5
6 3.67E-01 0 907.539 218.972 738.668 156.16 777 162 579 87
7 2.23E-01 0 315.178 189.566 285.844 181.403 223.5 123 210.5 118.5
8 -5.36E-01 0 341.26 199.533 412.962 181 243 127 315 74
9 2.43E-01 0 599.042 203.133 542.962 173.051 496 123 420 93
10 -8.12E-01 0 223.357 211.194 223.182 137.492 154 132 153 63
11 -4.92E-01 0 223.474 195.761 216.009 164.254 141 133 127 90
12 2.12E-02 0 214.39 173.542 199.11 160.124 141 90 120 75
13 1.10E+00 0 506.699 160.305 326.494 178.538 402 85 223 108
14 -2.12E-01 0 208.857 169.264 212.87 166.973 135 107 125 96
15 -2.02E-01 0 1025.06 201.322 1229.6 182.839 892 124 982 117.5
16 -2.83E-01 0 432.315 253.184 459.762 188.616 357 185 337 129
17 -2.46E-01 0 972.856 258.305 1125.11 198.557 903 197 959 114
18 5.02E-01 0 323.991 187.04 249.199 177.671 263 116.5 167 100
19 -2.31E-01 0 297.341 181.342 275.428 148.964 225 116 188 56
20 -4.16E-01 0 226.026 189.984 234.459 179.157 129 126 166 102

Total number of rows: 14720

Table truncated, full table size 943 Kbytes.




Supplementary data files not provided

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