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Status
Public on May 21, 2004
Title
B chip Version1: time 1 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
185.046
180.007
153.313
164.414
103
97.5
75.5
91.5
2
1.99E-01
0
216.719
171.246
172.146
165.101
141
105.5
79
75.5
3
9.28E-01
0
2344.32
204.221
1270.92
185.111
2072
127
957
118
4
-4.12E-01
0
190.63
190.208
173.007
164.531
124
122
98
77.5
5
8.17E-01
0
2330.58
179.068
1307.47
141.963
2097
112.5
1113
70.5
6
3.67E-01
0
907.539
218.972
738.668
156.16
777
162
579
87
7
2.23E-01
0
315.178
189.566
285.844
181.403
223.5
123
210.5
118.5
8
-5.36E-01
0
341.26
199.533
412.962
181
243
127
315
74
9
2.43E-01
0
599.042
203.133
542.962
173.051
496
123
420
93
10
-8.12E-01
0
223.357
211.194
223.182
137.492
154
132
153
63
11
-4.92E-01
0
223.474
195.761
216.009
164.254
141
133
127
90
12
2.12E-02
0
214.39
173.542
199.11
160.124
141
90
120
75
13
1.10E+00
0
506.699
160.305
326.494
178.538
402
85
223
108
14
-2.12E-01
0
208.857
169.264
212.87
166.973
135
107
125
96
15
-2.02E-01
0
1025.06
201.322
1229.6
182.839
892
124
982
117.5
16
-2.83E-01
0
432.315
253.184
459.762
188.616
357
185
337
129
17
-2.46E-01
0
972.856
258.305
1125.11
198.557
903
197
959
114
18
5.02E-01
0
323.991
187.04
249.199
177.671
263
116.5
167
100
19
-2.31E-01
0
297.341
181.342
275.428
148.964
225
116
188
56
20
-4.16E-01
0
226.026
189.984
234.459
179.157
129
126
166
102
Total number of rows: 14720 Table truncated, full table size 943 Kbytes .Supplementary data files not provided