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Sample GSM22773 Query DataSets for GSM22773
Status Public on May 21, 2004
Title B chip Version1: time 8 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 236.118 221.602 200.592 216.243 128.5 111.5 107 120
2 2.94E-01 0 323.479 221.992 257.501 202.217 199 112 141 111.5
3 4.74E-01 0 1598.77 247.496 856.647 227.707 1295 141.5 675 119
4 5.18E-01 0 279.39 256.656 222.761 238.83 154 136 113 126
5 3.48E-02 0 1921.61 234.715 1172.77 214.852 1778 130.5 960 102.5
6 4.19E-01 0 916.062 278.256 556.566 202.644 757 151 416 102
7 -4.39E-03 0 394.02 213.873 313.036 193.348 261 135 167 98
8 3.59E-01 0 384.25 233.189 280.518 252.811 234 140 173 118.5
9 2.24E-01 0 530.536 232.807 373.7 186.466 388.5 138 220.5 102.5
10 5.25E-01 0 274.678 238.269 230.517 218.488 149 118.5 123.5 127.5
11 1.16E-01 0 294.457 247.51 225.485 210.957 174 148 124 101
12 -1.38E-01 0 271.58 239.894 238.501 194.306 153 144.5 125 108.5
13 3.94E-01 0 357.646 212.54 247.8 201.968 231 116 131 91.5
14 -6.54E-01 0 278.196 281.112 270.66 211.91 164 171 157 116
15 7.56E-03 0 572.39 260.399 452.133 223.633 467 157 309 120
16 -2.07E-01 0 343.53 261.867 320.311 255.757 200 153 204 137
17 -1.18E-01 0 615.605 302.696 516.459 263.378 498 182 365 138
18 -6.02E-02 0 255.685 240.328 220.774 199.667 142 142.5 121 107.5
19 -2.69E-01 0 312.277 280.481 287.588 223.795 195 168 164 135
20 5.96E-01 0 330.676 255.133 253.43 239.807 196 148 148 143

Total number of rows: 14720

Table truncated, full table size 950 Kbytes.




Supplementary data files not provided

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