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Status
Public on May 21, 2004
Title
B chip Version1: time 8 hour Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
236.118
221.602
200.592
216.243
128.5
111.5
107
120
2
2.94E-01
0
323.479
221.992
257.501
202.217
199
112
141
111.5
3
4.74E-01
0
1598.77
247.496
856.647
227.707
1295
141.5
675
119
4
5.18E-01
0
279.39
256.656
222.761
238.83
154
136
113
126
5
3.48E-02
0
1921.61
234.715
1172.77
214.852
1778
130.5
960
102.5
6
4.19E-01
0
916.062
278.256
556.566
202.644
757
151
416
102
7
-4.39E-03
0
394.02
213.873
313.036
193.348
261
135
167
98
8
3.59E-01
0
384.25
233.189
280.518
252.811
234
140
173
118.5
9
2.24E-01
0
530.536
232.807
373.7
186.466
388.5
138
220.5
102.5
10
5.25E-01
0
274.678
238.269
230.517
218.488
149
118.5
123.5
127.5
11
1.16E-01
0
294.457
247.51
225.485
210.957
174
148
124
101
12
-1.38E-01
0
271.58
239.894
238.501
194.306
153
144.5
125
108.5
13
3.94E-01
0
357.646
212.54
247.8
201.968
231
116
131
91.5
14
-6.54E-01
0
278.196
281.112
270.66
211.91
164
171
157
116
15
7.56E-03
0
572.39
260.399
452.133
223.633
467
157
309
120
16
-2.07E-01
0
343.53
261.867
320.311
255.757
200
153
204
137
17
-1.18E-01
0
615.605
302.696
516.459
263.378
498
182
365
138
18
-6.02E-02
0
255.685
240.328
220.774
199.667
142
142.5
121
107.5
19
-2.69E-01
0
312.277
280.481
287.588
223.795
195
168
164
135
20
5.96E-01
0
330.676
255.133
253.43
239.807
196
148
148
143
Total number of rows: 14720 Table truncated, full table size 950 Kbytes .Supplementary data files not provided