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Status
Public on May 21, 2004
Title
B chip Version1: time 24 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
221.241
170.277
196.014
184.325
118
91.5
103.5
103.5
2
-1.98E-01
0
254.468
250.561
261.842
227.358
166
144
159
142
3
-5.36E-02
0
1198.24
245.441
1014.27
225.226
1038
126
801.5
130
4
3.17E-01
0
255.754
207.329
244.569
222.106
144
105
138
133
5
4.25E-01
0
2214.4
263.816
1225.24
223.984
1970
140
1033
112
6
2.63E-01
0
559.002
229.608
418.071
197.379
460
117
272
110
7
-4.63E-02
0
433.683
249.7
360.809
189.359
290
143.5
232
101
8
-3.00E-01
0
258.856
198.186
283.958
208.199
137
105
155
114
9
1.14E-02
0
362.604
234.452
330.919
210.528
255
126
209
124
10
6.09E-02
0
229.278
181.875
223.068
213.181
118
96
117
104.5
11
-1.82E-02
0
216.28
209.981
223.547
205.757
111
111
121
120
12
-3.76E-01
0
228.809
217.934
233.616
199.668
113
124.5
122
105.5
13
1.53E-01
0
284.733
189.028
241.526
185.426
170
104.5
126
96.5
14
5.26E-02
0
223.979
212.748
215.123
223.761
126
110
115
110
15
-4.74E-01
0
378.601
208.07
426.917
202.43
244
104
269
101
16
-5.34E-02
0
303.486
256.747
309.115
278.811
193
152
177
166
17
9.40E-02
0
541.583
231.305
446.652
220.727
426
123
295
118
18
2.70E-01
0
293.464
233.254
228.908
215.546
169
130.5
123.5
105.5
19
1.08E-01
0
273.698
214.738
242.636
215.748
149
122.5
134
114
20
2.64E-01
0
243.244
252.042
214.179
252.521
143
155
112
133
Total number of rows: 14720 Table truncated, full table size 949 Kbytes .Supplementary data files not provided