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Status
Public on May 21, 2004
Title
B chip Version1: time 24 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
243.143
193.489
197.394
167.264
145
105.5
101
89.5
2
-2.56E-01
0
281.067
233.484
250.466
188.17
166
132.5
136
96.5
3
1.27E-01
0
1369.79
324.569
908.492
237.431
1140
204
749
132
4
-4.74E-01
0
261.863
290.612
242.721
205.192
147.5
153
150
107
5
8.46E-01
0
2449.85
282.38
1019.18
218.481
2157.5
165
868.5
127
6
4.17E-01
0
624.885
265.806
415.421
217.372
510
142
280
124
7
3.95E-01
0
460.796
276.665
298.43
197.615
309
162.5
188
105.5
8
1.25E-01
0
344.682
239.333
300.036
246.013
234
128
178.5
127
9
1.32E-01
0
446.123
236.103
365.75
217.662
311
130
236.5
131.5
10
-2.11E-01
0
295.282
222.6
259.033
198.529
174
137.5
143
100
11
2.16E-01
0
302.854
236.931
233.757
189.682
175.5
125.5
124
99
12
-1.38E-02
0
267.916
265.639
229.819
222.148
160
150.5
132
120.5
13
3.59E-01
0
337.491
222.96
278.872
220.708
202.5
108.5
168
122
14
6.95E-01
0
294.133
232.398
234.377
219.008
180
112
134
132
15
-3.87E-01
0
504.632
243.505
479.282
204.668
397
136
342
101
16
9.82E-02
0
314.983
269.657
290.464
264.393
199
158
174
161
17
-3.04E-02
0
655.995
266.948
541.962
235.115
527.5
143
387
124.5
18
3.21E-01
0
328.089
266.227
247.109
234.11
203
163
132
129
19
3.69E-01
0
296.005
203.542
255.894
221.839
154
104
153
124
20
9.46E-02
0
257.719
218.706
230.013
214.077
151
111
138
107
Total number of rows: 14720 Table truncated, full table size 949 Kbytes .Supplementary data files not provided