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Sample GSM22779 Query DataSets for GSM22779
Status Public on May 21, 2004
Title B chip Version1: time 24 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1231
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 243.143 193.489 197.394 167.264 145 105.5 101 89.5
2 -2.56E-01 0 281.067 233.484 250.466 188.17 166 132.5 136 96.5
3 1.27E-01 0 1369.79 324.569 908.492 237.431 1140 204 749 132
4 -4.74E-01 0 261.863 290.612 242.721 205.192 147.5 153 150 107
5 8.46E-01 0 2449.85 282.38 1019.18 218.481 2157.5 165 868.5 127
6 4.17E-01 0 624.885 265.806 415.421 217.372 510 142 280 124
7 3.95E-01 0 460.796 276.665 298.43 197.615 309 162.5 188 105.5
8 1.25E-01 0 344.682 239.333 300.036 246.013 234 128 178.5 127
9 1.32E-01 0 446.123 236.103 365.75 217.662 311 130 236.5 131.5
10 -2.11E-01 0 295.282 222.6 259.033 198.529 174 137.5 143 100
11 2.16E-01 0 302.854 236.931 233.757 189.682 175.5 125.5 124 99
12 -1.38E-02 0 267.916 265.639 229.819 222.148 160 150.5 132 120.5
13 3.59E-01 0 337.491 222.96 278.872 220.708 202.5 108.5 168 122
14 6.95E-01 0 294.133 232.398 234.377 219.008 180 112 134 132
15 -3.87E-01 0 504.632 243.505 479.282 204.668 397 136 342 101
16 9.82E-02 0 314.983 269.657 290.464 264.393 199 158 174 161
17 -3.04E-02 0 655.995 266.948 541.962 235.115 527.5 143 387 124.5
18 3.21E-01 0 328.089 266.227 247.109 234.11 203 163 132 129
19 3.69E-01 0 296.005 203.542 255.894 221.839 154 104 153 124
20 9.46E-02 0 257.719 218.706 230.013 214.077 151 111 138 107

Total number of rows: 14720

Table truncated, full table size 949 Kbytes.




Supplementary data files not provided

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