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Status
Public on May 21, 2004
Title
B chip Version2: time 1 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
165.596
142.443
171.706
163.222
97
78
107
75.5
2
2.72E-02
0
196.125
179.697
225.432
194.292
133
106.5
157
138
3
1.08E+00
0
3920.69
323.816
2914.22
362.706
3935
269
2763
304
4
2.34E-01
0
319.164
244.852
302.658
243.044
264.5
175.5
238
172.5
5
5.59E-01
0
6015.55
274.615
5932.86
269.97
5969
217
5846
204.5
6
3.10E-01
0
651.601
227.163
801.341
263.411
593
177
685
207
7
9.07E-02
0
21107.4
485.927
17241.7
536.678
21503
452
17394
468
8
-5.03E-01
0
287.098
221.519
422.749
258.288
217
150
322.5
181.5
9
2.04E-01
0
333.656
186.767
361.319
182.333
257
112.5
289
109.5
10
5.74E-01
0
193.217
165.74
143.18
149.439
122.5
83.5
77
72.5
11
3.09E-02
0
152.649
113.462
160.269
147.504
79
59
95.5
68.5
12
3.92E-01
0
194.652
147.738
148.867
124.981
119
84
85
66
13
9.62E-01
0
584.158
169.928
453.524
139.52
521.5
91.5
357
82
14
-6.86E-03
0
178.428
135.564
156.28
137.367
109
88.5
97
66
15
-6.45E-01
0
743.878
196.589
1690.24
202.047
626
145
1579
141.5
16
-3.16E-01
0
401.428
203.929
636.129
235.884
321
152
559
159
17
-9.36E-02
0
1033.72
325.127
1648.12
342.117
942
227
1522
260
18
3.85E-01
0
350.159
213.571
334.623
227.929
275
145
268.5
144.5
19
-3.85E-01
0
234.836
202.645
244.131
137.783
165.5
126
159
88.5
20
-1.36E-01
0
224.563
176.009
216.946
157.076
148
115
161
90
Total number of rows: 14720 Table truncated, full table size 958 Kbytes .Supplementary data files not provided