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Sample GSM22838 Query DataSets for GSM22838
Status Public on May 21, 2004
Title B chip Version2: time 1 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 165.596 142.443 171.706 163.222 97 78 107 75.5
2 2.72E-02 0 196.125 179.697 225.432 194.292 133 106.5 157 138
3 1.08E+00 0 3920.69 323.816 2914.22 362.706 3935 269 2763 304
4 2.34E-01 0 319.164 244.852 302.658 243.044 264.5 175.5 238 172.5
5 5.59E-01 0 6015.55 274.615 5932.86 269.97 5969 217 5846 204.5
6 3.10E-01 0 651.601 227.163 801.341 263.411 593 177 685 207
7 9.07E-02 0 21107.4 485.927 17241.7 536.678 21503 452 17394 468
8 -5.03E-01 0 287.098 221.519 422.749 258.288 217 150 322.5 181.5
9 2.04E-01 0 333.656 186.767 361.319 182.333 257 112.5 289 109.5
10 5.74E-01 0 193.217 165.74 143.18 149.439 122.5 83.5 77 72.5
11 3.09E-02 0 152.649 113.462 160.269 147.504 79 59 95.5 68.5
12 3.92E-01 0 194.652 147.738 148.867 124.981 119 84 85 66
13 9.62E-01 0 584.158 169.928 453.524 139.52 521.5 91.5 357 82
14 -6.86E-03 0 178.428 135.564 156.28 137.367 109 88.5 97 66
15 -6.45E-01 0 743.878 196.589 1690.24 202.047 626 145 1579 141.5
16 -3.16E-01 0 401.428 203.929 636.129 235.884 321 152 559 159
17 -9.36E-02 0 1033.72 325.127 1648.12 342.117 942 227 1522 260
18 3.85E-01 0 350.159 213.571 334.623 227.929 275 145 268.5 144.5
19 -3.85E-01 0 234.836 202.645 244.131 137.783 165.5 126 159 88.5
20 -1.36E-01 0 224.563 176.009 216.946 157.076 148 115 161 90

Total number of rows: 14720

Table truncated, full table size 958 Kbytes.




Supplementary data files not provided

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