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Status
Public on May 21, 2004
Title
B chip Version2: time 8 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
227.889
214.377
130.65
163.417
141
136
58
91
2
-5.01E-01
0
313.208
261.038
180.1
152.753
266
189
104
54.5
3
7.98E-01
0
2583.7
331.906
847.581
198.765
2443
258
740
125
4
5.57E-01
0
460.615
269.083
223.469
182.738
383
164.5
144
98
5
4.16E-01
0
12085.5
370.372
3959.71
218.43
12351
312
3881
152
6
6.24E-01
0
1442.28
296.671
550.106
198.298
1301
222
475
141
7
-2.73E-01
0
27626.8
473.923
9200.62
306.753
31282
413
9930
210
8
3.24E-01
0
439.889
338.692
235.623
204.968
353
271
149
139
9
5.50E-01
0
538.397
200.657
245.805
165.696
445
107
136
75
10
-1.64E-01
0
257.639
230.258
150.639
150.314
179
150.5
79
56.5
11
2.93E-01
0
240.503
212.139
131.442
143.571
177.5
118
57
52
12
-2.26E-01
0
275.029
211.992
176.027
131.708
179.5
130
99
56.5
13
1.09E+00
0
871.973
203.586
251.521
131.162
740
141.5
148
58.5
14
NULL
0
229.533
207.867
167.518
150.129
163
156
84
75
15
2.24E-01
0
783.903
221.737
381.138
159.108
686
167
291
94
16
-4.55E-01
0
420.683
300.479
250.517
165.042
348
230.5
191
85.5
17
-1.32E-01
0
1218.82
324.243
646.681
204.318
1084
275
566
117
18
4.15E-01
0
960.656
264.954
411.843
179.915
825
182
327
102
19
2.17E-01
0
353.111
261.952
225.652
188.884
276
203
151
131
20
-3.61E-01
0
384.506
260.795
281.916
227.434
308
213
230
136.5
Total number of rows: 14720 Table truncated, full table size 953 Kbytes .Supplementary data files not provided