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Sample GSM22848 Query DataSets for GSM22848
Status Public on May 21, 2004
Title B chip Version2: time 24 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 253.989 242.172 244.641 220.942 165 181 165 151
2 NULL 0 284.488 259.713 299.821 309.678 221 214 243 254.5
3 1.41E-01 0 2926.79 391.459 3411.08 552.678 2869 300 3387 528
4 -1.89E-02 0 378.53 297.938 435.009 358.353 327 245 395 300
5 2.44E-01 0 9463.46 333.848 9037.69 382.918 10202 289.5 9652 294
6 1.41E-01 0 1214.85 340.815 1290.49 366.926 1097 289.5 1129 339.5
7 -3.41E-01 0 24132.6 502.078 21446.7 641.576 27634 458 24205 596
8 -5.43E-01 0 382.026 308.403 501.184 367.517 295 250.5 444 309
9 4.82E-02 0 560.005 252.23 596.128 247.389 431 167 471 182
10 NULL 0 275.444 238.864 262.111 231.548 198 173 196 173.5
11 NULL 0 236.45 232.483 249.68 252.839 169 146.5 180 185.5
12 NULL 0 260.373 238.052 235.955 184.42 185 152 179 110.5
13 8.15E-01 0 1069.85 207.18 776.319 236.498 866 127 623 175
14 NULL 0 233.845 230.3 250.326 249.739 155 158 195 178
15 -2.21E-01 0 936.308 207.493 1256.4 273.991 786 131 1050 199
16 -5.24E-01 0 381.404 224.12 552.073 313.089 306 153 483 223
17 -2.76E-01 0 1193.63 331.279 1657.89 490.399 1059 284.5 1501 392.5
18 4.59E-02 0 573.275 214.869 662.694 291.151 481 147.5 574 206.5
19 NULL 0 312.772 252.401 394.217 255 236 165.5 339 223.5
20 -2.69E-02 0 370.806 309.689 375.514 270.425 300 219.5 317.5 220

Total number of rows: 14720

Table truncated, full table size 953 Kbytes.




Supplementary data files not provided

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