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Status
Public on May 21, 2004
Title
B chip Version2: time 24 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
253.989
242.172
244.641
220.942
165
181
165
151
2
NULL
0
284.488
259.713
299.821
309.678
221
214
243
254.5
3
1.41E-01
0
2926.79
391.459
3411.08
552.678
2869
300
3387
528
4
-1.89E-02
0
378.53
297.938
435.009
358.353
327
245
395
300
5
2.44E-01
0
9463.46
333.848
9037.69
382.918
10202
289.5
9652
294
6
1.41E-01
0
1214.85
340.815
1290.49
366.926
1097
289.5
1129
339.5
7
-3.41E-01
0
24132.6
502.078
21446.7
641.576
27634
458
24205
596
8
-5.43E-01
0
382.026
308.403
501.184
367.517
295
250.5
444
309
9
4.82E-02
0
560.005
252.23
596.128
247.389
431
167
471
182
10
NULL
0
275.444
238.864
262.111
231.548
198
173
196
173.5
11
NULL
0
236.45
232.483
249.68
252.839
169
146.5
180
185.5
12
NULL
0
260.373
238.052
235.955
184.42
185
152
179
110.5
13
8.15E-01
0
1069.85
207.18
776.319
236.498
866
127
623
175
14
NULL
0
233.845
230.3
250.326
249.739
155
158
195
178
15
-2.21E-01
0
936.308
207.493
1256.4
273.991
786
131
1050
199
16
-5.24E-01
0
381.404
224.12
552.073
313.089
306
153
483
223
17
-2.76E-01
0
1193.63
331.279
1657.89
490.399
1059
284.5
1501
392.5
18
4.59E-02
0
573.275
214.869
662.694
291.151
481
147.5
574
206.5
19
NULL
0
312.772
252.401
394.217
255
236
165.5
339
223.5
20
-2.69E-02
0
370.806
309.689
375.514
270.425
300
219.5
317.5
220
Total number of rows: 14720 Table truncated, full table size 953 Kbytes .Supplementary data files not provided