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Status
Public on May 21, 2004
Title
B chip Version2: time 3 day Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
222.721
222.258
156.173
152.72
153
158.5
84
74.5
2
NULL
0
267.572
248.209
188.359
163.004
199
171.5
118
95
3
-4.05E-01
0
683.86
254.389
1371.93
228.955
579
208
1282
152
4
4.07E-01
0
292.975
222.159
238.269
190.112
217
164
149
124.5
5
3.57E-01
0
3420.16
258.228
4923.81
222.398
3419
219.5
5060
148
6
-1.00E+00
0
354.111
260.753
545.572
183.662
288
212
440
92
7
-1.14E-02
0
11287
318.594
15003.7
354.382
11879
270
16626
310
8
NULL
0
283.548
194.601
231.258
227.929
197
143
140
145
9
-2.69E-01
0
247.86
195.552
263.627
162.74
172
127
184
89
10
NULL
0
214.946
222.452
143.449
163.384
138
156
69
80
11
NULL
0
203.517
207.016
128.683
151.172
135
110.5
55
74
12
5.61E-01
0
230.305
171.216
154.581
91.9478
156
83.5
83
36.5
13
-7.52E-01
0
280.658
233.53
319.665
140.257
200
160
247
47
14
NULL
0
202.857
194.086
153.287
151.179
143
137
78.5
75.5
15
-9.05E-02
0
392.801
206.304
575.913
165.346
310
131
465
77
16
NULL
0
250.574
209.982
237.827
162.859
184
151
155
87
17
5.05E-02
0
441.461
244.131
608.803
223.653
380
165
516
130
18
5.51E-02
0
257.634
204.743
203.42
167.703
198
147
134
79.5
19
NULL
0
259.464
199.699
181.13
139.611
184.5
118
99.5
83.5
20
NULL
0
253.572
250.671
166.694
161.638
194
182
88
69
Total number of rows: 14720 Table truncated, full table size 935 Kbytes .Supplementary data files not provided