NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22849 Query DataSets for GSM22849
Status Public on May 21, 2004
Title B chip Version2: time 3 day Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 222.721 222.258 156.173 152.72 153 158.5 84 74.5
2 NULL 0 267.572 248.209 188.359 163.004 199 171.5 118 95
3 -4.05E-01 0 683.86 254.389 1371.93 228.955 579 208 1282 152
4 4.07E-01 0 292.975 222.159 238.269 190.112 217 164 149 124.5
5 3.57E-01 0 3420.16 258.228 4923.81 222.398 3419 219.5 5060 148
6 -1.00E+00 0 354.111 260.753 545.572 183.662 288 212 440 92
7 -1.14E-02 0 11287 318.594 15003.7 354.382 11879 270 16626 310
8 NULL 0 283.548 194.601 231.258 227.929 197 143 140 145
9 -2.69E-01 0 247.86 195.552 263.627 162.74 172 127 184 89
10 NULL 0 214.946 222.452 143.449 163.384 138 156 69 80
11 NULL 0 203.517 207.016 128.683 151.172 135 110.5 55 74
12 5.61E-01 0 230.305 171.216 154.581 91.9478 156 83.5 83 36.5
13 -7.52E-01 0 280.658 233.53 319.665 140.257 200 160 247 47
14 NULL 0 202.857 194.086 153.287 151.179 143 137 78.5 75.5
15 -9.05E-02 0 392.801 206.304 575.913 165.346 310 131 465 77
16 NULL 0 250.574 209.982 237.827 162.859 184 151 155 87
17 5.05E-02 0 441.461 244.131 608.803 223.653 380 165 516 130
18 5.51E-02 0 257.634 204.743 203.42 167.703 198 147 134 79.5
19 NULL 0 259.464 199.699 181.13 139.611 184.5 118 99.5 83.5
20 NULL 0 253.572 250.671 166.694 161.638 194 182 88 69

Total number of rows: 14720

Table truncated, full table size 935 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap