GEO help: Mouse over screen elements for information.
Status
Public on May 21, 2004
Title
B chip Version2: time 5 day Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
231.585
183.29
199.208
195.102
114
99
110
102
2
0.030175638
0
270.544
232.759
231.785
189.151
162
120.5
139
94.5
3
0.463552345
0
1758.32
240.125
1124.41
215.576
1402
126
855
119.5
4
-0.060298902
0
263.632
228.344
240.299
189.861
148
121
131
102
5
0.206981842
0
904.962
255.065
700.495
217.155
770
140
626
119
6
0.298771417
0
688.63
240.534
499.494
181.566
560
132
351
100
7
-0.189641293
0
289.313
231.973
265.814
182.469
174
135
154
95
8
0.697659096
0
321.371
197.529
247.522
194.931
187
104
137
120
9
0.398132969
0
553.399
258.207
414.026
208.385
447
132
275
131.5
10
0.658269881
0
278.599
221.191
220.719
223.689
155
122
121
130
11
0.089150639
0
259.834
211.606
227.406
204.458
155
115
118
103
12
0.688173258
0
259.225
246.333
223.674
272.267
149
115.5
116
140.5
13
0.593347176
0
385.383
245.695
276.169
213.68
244
125
158
117
14
-0.447931804
0
264.454
275.589
214.816
182.636
169
174
121
96.5
15
0.033807828
0
657.969
225.353
575.182
213.408
532
127
433
115
16
-0.406754624
0
337.269
205.479
396.438
217.988
212
98
258
109
17
0.329563437
0
746.024
253.507
566.022
257.144
630
137.5
412
139
18
0.229203074
0
309.176
251.122
235.663
192.912
200
143
125
102
19
0.205756194
0
318.82
252.676
252.317
209.933
194
146.5
137
108.5
20
0.080048098
0
246.547
214.938
234.763
252.366
141
133.5
127.5
133.5
Total number of rows: 14720 Table truncated, full table size 992 Kbytes .Supplementary data files not provided