NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22858 Query DataSets for GSM22858
Status Public on May 21, 2004
Title B chip Version2: time 5 day Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 231.585 183.29 199.208 195.102 114 99 110 102
2 0.030175638 0 270.544 232.759 231.785 189.151 162 120.5 139 94.5
3 0.463552345 0 1758.32 240.125 1124.41 215.576 1402 126 855 119.5
4 -0.060298902 0 263.632 228.344 240.299 189.861 148 121 131 102
5 0.206981842 0 904.962 255.065 700.495 217.155 770 140 626 119
6 0.298771417 0 688.63 240.534 499.494 181.566 560 132 351 100
7 -0.189641293 0 289.313 231.973 265.814 182.469 174 135 154 95
8 0.697659096 0 321.371 197.529 247.522 194.931 187 104 137 120
9 0.398132969 0 553.399 258.207 414.026 208.385 447 132 275 131.5
10 0.658269881 0 278.599 221.191 220.719 223.689 155 122 121 130
11 0.089150639 0 259.834 211.606 227.406 204.458 155 115 118 103
12 0.688173258 0 259.225 246.333 223.674 272.267 149 115.5 116 140.5
13 0.593347176 0 385.383 245.695 276.169 213.68 244 125 158 117
14 -0.447931804 0 264.454 275.589 214.816 182.636 169 174 121 96.5
15 0.033807828 0 657.969 225.353 575.182 213.408 532 127 433 115
16 -0.406754624 0 337.269 205.479 396.438 217.988 212 98 258 109
17 0.329563437 0 746.024 253.507 566.022 257.144 630 137.5 412 139
18 0.229203074 0 309.176 251.122 235.663 192.912 200 143 125 102
19 0.205756194 0 318.82 252.676 252.317 209.933 194 146.5 137 108.5
20 0.080048098 0 246.547 214.938 234.763 252.366 141 133.5 127.5 133.5

Total number of rows: 14720

Table truncated, full table size 992 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap