NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2331300 Query DataSets for GSM2331300
Status Public on Sep 01, 2018
Title PCOS 154A
Sample type RNA
 
Source name PCOS, ovary sample
Organism Homo sapiens
Characteristics gender: female
disease state: PCOS
tissue: ovary
Treatment protocol There was no treatment on the tissues.
Growth protocol All tissue samples were snap frozen in liquid nitrogen immediately after collection.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from Ovary biopsies using TRIZOL reagent (Invitrogen, Breda, The Netherlands) as per manufacturer's instructions. After Trizol purification, RNA was repurified using the Rneasy Mini Kit (Qiagen, Venlo., The Netherlands) according to manufacturer's instructions. Total RNA was quantified by OD260/OD280 measurement and with RiboGreen RNA Quantitation Kit (Moleular Probes, Leiden, The Netherlands). RNA quality was assessed on a 6000 nanochip using Agilent 2100 bioanalyzer (Agilent Technologys, Waldbronn, Germany).
Label biotin
Label protocol cRNA was biotin-labeled.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocols.
Scan protocol Chips were scanned on an Affymetrix Scanner according to the manufacturers protocol.
Description Gene expression data from ovary biopt material
Data processing The data were analyzed with Partek Genomics Suite version 6.6 using GCRMA with default settings and quantile normalization method. The data is log2 transformed.
 
Submission date Sep 28, 2016
Last update date Sep 01, 2018
Contact name Arne IJpma
Organization name Erasmus MC
Department Bioinformatics
Street address Dr. Molewaterplein 50
City Rotterdam
ZIP/Postal code 3000 CA
Country Netherlands
 
Platform ID GPL96
Series (1)
GSE87435 Microarray expression data derived from ovaries of women with Polycystic Ovary Syndrome and Female to Male Transexual individuals after treatment with Testosterone

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity

Data table
ID_REF VALUE
1007_s_at 8.64734
1053_at 5.89939
117_at 3.10588
121_at 6.60046
1255_g_at 2.39818
1294_at 7.38681
1316_at 3.63399
1320_at 3.30797
1405_i_at 4.17329
1431_at 2.9865
1438_at 2.51317
1487_at 3.9506
1494_f_at 2.55978
1598_g_at 9.19027
160020_at 5.84219
1729_at 5.69326
1773_at 1.86646
177_at 6.92201
179_at 1.75117
1861_at 1.28276

Total number of rows: 22283

Table truncated, full table size 409 Kbytes.




Supplementary file Size Download File type/resource
GSM2331300_000154_HGU133A.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap