|
| Status |
Public on Jul 01, 2018 |
| Title |
pair 12, diagnosis Schiz, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
pair 12_diagnosis Schiz
|
| Organism |
Homo sapiens |
| Characteristics |
pair id: pair 12
dsm-iv diagnoses: Schizoaffective disorder; ADR
subject group: schizophrenia
tissue: dorsolateral prefrontal cortex (DLPFC)
cell type: parvalbumin (PV) neurons
|
| Treatment protocol |
An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
|
| Growth protocol |
Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
|
| Label |
Encore Biotin module (Nugen)
|
| Label protocol |
Labeled using the Encore Biotin module (Nugen).
|
| |
|
| Hybridization protocol |
CDNA was synthesized and amplified using the Ovation Pico WTA System.
|
| Scan protocol |
Loaded on an Affymetrix GeneChip® U219.
|
| Description |
Schiz_539_2
|
| Data processing |
Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
|
| |
|
| Submission date |
Jan 12, 2017 |
| Last update date |
Jul 01, 2018 |
| Contact name |
David Lewis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Pittsburgh
|
| Department |
Psychiatry and Neuroscience
|
| Street address |
3811 O'Hara St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE93577 |
Gene expression of L3 parvalbumin neurons in the DLPFC comparing schizophrenia with unaffected subjects |
|
