|
Status |
Public on Aug 31, 2017 |
Title |
FFPE_X219016692 [X89_X.172_plate2NHSII] |
Sample type |
RNA |
|
|
Source name |
breast tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast tumor-adjacent normal age at diagnosis: 44.91666667 year of diagnosis: 1998 estrogen receptor status: Negative alcohol intake: 0-10 g/day microarray batch: 14 patient id: X219016692 nhs_cohort: NHS2 bmi_2yrs_bef_dx: 29.95 avg_bodysize_age1020: 3
|
Treatment protocol |
Formalin-fixed paraffin-embedded tissue blocks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen AllPrep RNA isolation kit was used to isolate total RNA.
|
Label |
biotin
|
Label protocol |
Labeling was performed according to standard Affymetrix protocols.
|
|
|
Hybridization protocol |
Hybridization was performed according to standard protocols.
|
Scan protocol |
Scanning was performed according to standard protocols.
|
Data processing |
Gene expression data were normalized and summarized using Robust Multiarray Average (RMA; Affymetrix Power Tools [APT] v1.18.0). Differential expression was determined using Limma with multiple hypothesis testing (Benjamini and Hochberg); Bioconductor, R. GSE93601_ER*_tumor_*_alcohol.xlsx: Limma analyses outputs.
|
|
|
Submission date |
Jan 13, 2017 |
Last update date |
Aug 20, 2020 |
Contact name |
Rulla M Tamimi |
E-mail(s) |
[email protected]
|
Phone |
617-525-0862
|
Organization name |
Brigham and Women's Hospital
|
Department |
Medicine
|
Lab |
Channing Division of Network Medicine
|
Street address |
181 Longwood Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL22920 |
Series (1) |
GSE93601 |
Alcohol Consumption and Breast Tumor Gene Expression |
|
Relations |
Reanalyzed by |
GSM3183580 |