|
| Status |
Public on Jun 24, 2024 |
| Title |
Kasumi-3 |
| Sample type |
RNA |
| |
|
| Source name |
non-AMKL_cultured AML cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Kasumi-3
cell type: Cultured AML cell line
cell subtype: non-AMKL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 200ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
|
| Description |
Gene expression data from cultured acute myeloid leukemia cell line.
|
| Data processing |
Data were normalized by Robust Multichip Average (RMA) using R 3.3.1, Bioconductor Affy package ver 1.5
|
| |
|
| Submission date |
May 05, 2017 |
| Last update date |
Jun 24, 2024 |
| Contact name |
Tuan Zea Tan |
| E-mail(s) |
[email protected]
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Street address |
14 Medical Drive, #11-01, Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE98578 |
Expression data from cultured human acute megokaryblastic myeloid leukemia and acute myeloid leukemia cell lines |
|
