|
| Status |
Public on Jun 01, 2018 |
| Title |
Cultured cell line with HCV treated with brusatol rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HPI-Brl60
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Hepatoma cell line
|
| Treatment protocol |
Bruratol was administered at the concentration of GI50 after precultuted cell.
|
| Growth protocol |
The cells were maintained in DMEM with 10% FBS for 1 dyay as precultue and for 2 days after the administration of brusatol prior to RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of the cultured cell was exracted by using RNaasy Mini Kit (QIAGEN) according to the pmanufacture's protocol.
|
| Label |
Cy5
|
| Label protocol |
cDNA and Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Total RNA was extracted
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
May 15, 2017 |
| Last update date |
Jun 01, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13915 |
| Series (1) |
| GSE98920 |
Dual effect of the Nrf2 inhibitor for inhibition of HCV and cell proliferation |
|
