Fat from NOD mice with blood glucose level of >300mg/dl for 3 consecutive days.
Extracted molecule
total RNA
Extraction protocol
Trizol
Label
biotin
Label protocol
Affymetrix single cycle protocol.
Hybridization protocol
As described by Affymetrix. Standard protocol.
Scan protocol
Affymetrix GeneChip® Operating Software (GCOS) automates the control of GeneChip® Fluidics Stations and Scanners. In addition, GCOS acquires data, manages sample and experimental information, and performs gene expression data analysis.
Description
Fat from NOD mice with blood glucose level of >300mg/dl for 3 consecutive days.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
