|
| Status |
Public on Oct 31, 2018 |
| Title |
IRE1_L4440_1 |
| Sample type |
RNA |
| |
|
| Source name |
Larval stage L1 to young adult, RNAi
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype/variation: IRE1 knockout
developmental stage: young adult
RNAi: pL4440 (Empty vector)
|
| Treatment protocol |
Worm growth medium contains 25µg/mL carbenicillin and 1mM IPTG, grown from synchronised L1 stage to L4/young adult stage)
25µg/mL carbenicillin, 1mM IPTG, 25µg/mL Tunicamycin
|
| Growth protocol |
Synchronised C. elegans at L1 stage were grown on normal growth media with 25µg/mL carbenicillin and 1mM IPTG and harvested when most of the animals had reached young adulthood.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol and purified with QIAGEN RNeasy Columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using the Affymetrix WT PLUS Reagent Kit from 300ng total RNA
|
| |
|
| Hybridization protocol |
3 ug of fragmented and labeled cDNA were hybridized for 16 hrs at 45C on GeneChip C elegans ST arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
Data was processed using RMA
Normalized differential gene expression files on Series record
The resulting CHP files are generated by the Affymetrix Tiling Analysis Software (v. 3.0), where gene expression of untreated samples are compared against the pmt-2(RNAi) treated samples.
|
| |
|
| Submission date |
Jun 07, 2017 |
| Last update date |
Oct 31, 2018 |
| Contact name |
Guillaume Thibault |
| E-mail(s) |
[email protected]
|
| Phone |
65 6592 1787
|
| Organization name |
Nanyang Technological University
|
| Department |
School of Biological Sciences
|
| Lab |
Thibault Lab
|
| Street address |
60 Nanyang Drive
|
| City |
Singapore |
| State/province |
Outside the US or Canada |
| ZIP/Postal code |
637551 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL19230 |
| Series (1) |
| GSE99763 |
Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress |
|
