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Sample GSM27233 Query DataSets for GSM27233
Status Public on Nov 01, 2005
Title ceg1.4
Sample type RNA
 
Channel 1
Source name OY163 (ceg1-250) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 99
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R� (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R� (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2560 26430 100 48 49 13 28 28 4 88 82 0 12565 11146 5995 34 34 4 100 100 0 0.002 0.002 0.025 0.021 2.51 0.002 0.71 80 388 12551 11133 -9.291 20 12531 21 11112 3883 891701 5.25 2778 0 0
2 2730 26430 80 34 35 7 28 28 5 50 32 0 134 146 78 34 35 5 100 100 0 0.06 0.063 0.064 0.063 2.154 0.061 0.413 52 297 106 119 -4.059 6 100 7 112 1831 7609 1.4 22.2 0 0
3 2910 26420 90 45 45 10 28 29 5 80 71 0 74 75 19 33 34 5 100 96 0 0.415 0.405 0.372 0.353 2.659 0.363 0.444 52 361 58 59 -1.27 17 41 17 42 2342 3892 3.2 8.2 0 0
4 3080 26410 100 57 57 9 29 29 5 100 98 0 86 86 15 34 35 6 100 98 0 0.538 0.538 0.518 0.525 1.453 0.496 0.734 80 428 80 80 -0.893 28 52 28 52 4533 6900 5.6 8.5 0 0
5 3270 26420 100 53 52 9 29 29 5 96 92 0 70 68 13 35 35 5 98 93 0 0.686 0.697 0.679 0.715 1.678 0.666 0.652 80 429 59 56 -0.544 24 35 23 33 4179 5441 4.6 6.6 0 0
6 3440 26410 100 2191 2216 547 29 31 14 100 100 0 4824 4585 1057 35 37 24 100 100 0 0.451 0.481 0.468 0.485 1.333 0.472 0.934 80 417 6951 6737 -1.147 2162 4789 2187 4550 177279 366819 156.071 189.5 0 0
7 3620 26410 100 273 270 63 30 32 14 100 100 0 647 624 115 35 37 24 100 100 0 0.397 0.407 0.412 0.401 1.184 0.403 0.95 80 420 855 829 -1.333 243 612 240 589 21620 49889 17 24.458 0 0
8 3790 26400 110 96 95 18 29 30 6 100 100 0 394 384 64 34 35 5 100 100 0 0.186 0.189 0.19 0.186 1.296 0.176 0.875 80 460 427 416 -2.426 67 360 66 350 7623 30727 10.833 69.8 0 0
9 3970 26410 100 7561 7290 1610 29 29 4 100 100 0 1514 1444 324 35 36 10 100 100 0 5.093 5.153 5.088 5.127 1.26 5.298 0.946 80 415 9011 8670 2.348 7532 1479 7261 1409 583179 115540 1815.25 140.8 0 0
10 4140 26400 100 194 188 35 27 28 7 100 100 0 125 123 21 34 36 18 100 96 0 1.835 1.809 1.802 1.819 1.27 1.832 0.906 80 405 258 250 0.876 167 91 161 89 15036 9848 22.857 4.833 0 0
11 4320 26400 100 51 50 12 27 27 4 93 87 0 63 64 14 33 34 5 98 92 0 0.8 0.742 0.789 0.706 2.123 0.707 0.575 80 420 54 54 -0.322 24 30 23 31 4006 5099 5.75 6 0 0
12 4490 26390 110 143 155 57 27 27 3 100 100 0 96 106 32 33 34 5 100 100 0 1.841 1.753 1.729 1.753 1.448 1.841 0.837 80 463 179 201 0.881 116 63 128 73 12427 8487 42.667 14.4 0 0
13 4670 26390 100 53 54 13 27 27 4 98 95 0 80 83 22 34 34 5 98 97 0 0.565 0.551 0.547 0.549 1.795 0.534 0.707 80 432 72 76 -0.823 26 46 27 49 4354 6654 6.75 9.8 0 0
14 4850 26400 110 35 36 5 27 27 3 88 71 0 76 76 15 34 34 4 97 97 0 0.19 0.214 0.211 0.213 1.988 0.177 0.394 80 468 50 51 -2.392 8 42 9 42 2867 6052 3 10.5 0 0
15 5020 26380 90 113 115 28 27 27 3 100 100 0 61 61 12 34 34 4 96 94 0 3.185 3.259 3.253 3.25 1.604 3.53 0.692 52 354 113 115 1.671 86 27 88 27 5996 3185 29.333 6.75 0 0
16 5190 26370 90 58 59 14 27 27 3 100 100 0 47 49 9 33 34 5 86 71 0 2.214 2 2.327 2.214 2.474 2.331 0.421 52 381 45 48 1.147 31 14 32 16 3065 2543 10.667 3 0 0
17 5360 26370 70 35 35 5 27 27 4 71 46 0 43 42 5 34 35 5 75 21 0 0.889 1 1 0.884 2.446 0.609 0.142 32 236 17 16 -0.17 8 9 8 8 1113 1356 2 1.4 -50 0
18 5540 26380 80 35 36 7 27 27 3 75 61 0 40 41 6 34 34 5 53 30 0 1.333 1.286 1.245 1.14 2.682 1.077 0.147 52 296 14 16 0.415 8 6 9 7 1862 2112 3 1.4 0 0
19 5700 26360 90 65 65 9 27 27 3 100 100 0 53 53 10 33 34 5 96 86 0 1.9 1.9 1.895 2.015 1.736 1.869 0.577 52 365 58 58 0.926 38 20 38 20 3397 2767 12.667 3.8 0 0
20 5870 26340 80 37 37 6 27 28 3 86 71 0 45 46 7 34 34 5 82 55 0 0.909 0.833 0.786 0.779 2.325 0.708 0.222 52 325 21 22 -0.138 10 11 10 12 1925 2390 3 2.4 0 0

Total number of rows: 5760

Table truncated, full table size 967 Kbytes.




Supplementary data files not provided

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