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Sample GSM27240 Query DataSets for GSM27240
Status Public on Nov 01, 2005
Title spt5.194.3
Sample type RNA
 
Channel 1
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name GHY379 (spt5-194) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 66
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R? (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R? (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2530 25990 90 68 74 32 60 61 13 30 13 0 9162 7998 3633 147 149 20 100 100 0 0.001 0.002 0.014 0.013 1.201 0.002 0.106 52 347 9023 7865 8 9015 14 7851 3857 415870 1 392.45 0 0
2 2710 25970 100 64 67 16 60 63 26 13 1 0 190 309 251 143 146 21 66 53 0 0.085 0.042 0.07 0.12 4.038 0.039 0.142 80 425 51 173 -3.555 4 47 7 166 5381 24737 0.154 7.762 -100 0
3 2880 25970 100 68 72 29 61 63 27 10 3 0 160 165 24 138 140 19 52 30 0 0.318 0.407 0.446 0.465 3.047 0.659 0.139 80 404 29 38 -1.652 7 22 11 27 5796 13213 0.333 1.316 -50 0
4 3050 26000 100 218 232 88 61 63 13 100 100 0 236 238 23 139 142 19 100 100 0 1.619 1.727 1.618 1.643 1.472 2.174 0.627 80 402 254 270 0.695 157 97 171 99 18567 19009 13 5.053 0 0
5 3230 25970 100 87 90 38 59 61 15 58 45 0 133 137 38 104 102 34 43 16 0 0.966 0.939 0.882 0.732 2.629 0.682 0.334 80 430 57 64 -0.051 28 29 31 33 7221 10944 1.933 1.029 -50 0
6 3400 26000 90 6915 6654 1120 56 73 134 100 100 0 7893 7888 1492 51 59 44 100 100 0 0.875 0.842 0.837 0.847 1.103 0.833 0.965 52 347 14701 14435 -0.193 6859 7842 6598 7837 345987 410166 49.112 177.932 0 0
7 3580 25990 100 459 447 95 55 68 118 97 92 0 744 698 179 41 46 38 97 97 0 0.575 0.597 0.595 0.624 1.396 0.581 0.942 80 442 1107 1049 -0.799 404 703 392 657 35769 55812 3.212 17.158 0 0
8 3760 26000 110 1555 1543 137 55 55 13 100 100 0 1775 1740 199 39 40 6 100 100 0 0.864 0.875 0.877 0.878 1.15 0.873 0.963 80 475 3236 3189 -0.211 1500 1736 1488 1701 123447 139197 114.462 283.333 0 0
9 3940 25990 100 3789 3483 1173 56 57 13 100 100 0 6717 6114 2658 39 41 10 100 100 0 0.559 0.564 0.583 0.605 1.312 0.534 0.961 80 419 10411 9502 -0.839 3733 6678 3427 6075 278642 489139 263.538 607.3 0 0
10 4110 26000 100 495 484 98 55 56 13 100 100 0 491 459 117 40 41 6 100 100 0 0.976 1.024 1.01 1.062 1.377 1.017 0.927 80 387 891 848 -0.036 440 451 429 419 38748 36716 32.923 69.667 0 0
11 4280 26000 100 105 116 43 56 56 13 95 86 0 77 80 19 41 42 6 97 96 0 1.361 1.538 1.257 1.377 2.103 2.168 0.529 80 425 85 99 0.445 49 36 60 39 9245 6421 4.615 6.333 0 0
12 4460 26000 100 336 358 91 57 57 13 100 100 0 310 328 86 41 42 8 100 100 0 1.037 1.049 1.034 1.049 1.231 1.072 0.894 80 413 548 588 0.053 279 269 301 287 28671 26263 23.154 35.75 0 0
13 4650 26000 100 138 156 79 57 57 12 100 98 0 108 114 31 40 41 7 100 100 0 1.191 1.338 1.233 1.195 1.688 1.641 0.512 80 412 149 173 0.252 81 68 99 74 12445 9088 8.25 10.429 0 0
14 4800 25990 110 125 130 32 56 58 14 98 98 0 98 97 22 41 42 6 98 97 0 1.211 1.321 1.302 1.339 1.571 1.419 0.729 80 435 126 130 0.276 69 57 74 56 10439 7779 5.143 9.167 0 0
15 4980 25980 100 209 202 50 57 59 13 100 98 0 273 262 65 41 42 6 100 98 0 0.655 0.656 0.677 0.67 1.515 0.662 0.853 80 442 384 366 -0.61 152 232 145 221 16192 20951 11 36.667 0 0
16 5150 25980 90 97 129 128 59 61 14 92 63 0 77 85 31 41 42 7 98 94 0 1.056 1.591 1.294 1.141 1.967 3.352 0.613 52 384 74 114 0.078 38 36 70 44 6722 4444 4.857 6.143 -100 0
17 5330 26010 70 89 97 29 59 62 16 75 50 0 73 76 20 43 47 16 78 43 0 1 1.152 0.974 1.03 2.181 1.932 0.337 32 216 60 71 0 30 30 38 33 3108 2427 2.188 1.813 0 0
18 5500 25970 100 61 67 26 59 61 14 23 10 0 48 51 14 42 43 9 41 20 0 0.333 0.889 1.6 1.324 3.391 3.225 0.2 80 470 8 17 -1.585 2 6 8 9 5369 4100 0.429 0.889 -50 0
19 5710 25990 100 237 246 66 59 60 13 100 100 0 260 256 62 42 42 6 100 100 0 0.817 0.874 0.844 0.874 1.384 0.882 0.863 80 438 396 401 -0.292 178 218 187 214 19660 20518 14.308 35.667 0 0
20 5850 25990 90 97 109 47 60 60 14 86 65 0 78 83 23 42 42 6 100 98 0 1.028 1.195 1.012 0.988 1.95 1.711 0.573 52 303 73 90 0.04 37 36 49 41 5683 4293 3.5 6.833 0 0

Total number of rows: 5760

Table truncated, full table size 1006 Kbytes.




Supplementary data files not provided

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