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Sample GSM27241 Query DataSets for GSM27241
Status Public on Nov 01, 2005
Title spt5.194.4
Sample type RNA
 
Channel 1
Source name GHY379 (spt5-194) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 94
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R� (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R� (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2190 25680 90 57 59 10 31 32 7 98 94 0 11822 11140 3607 39 41 12 100 100 0 0.002 0.003 0 0 1 0.002 0.704 52 380 11809 11129 -8.824 26 11783 28 11101 3042 579267 3.857 924.917 0 0
2 2370 25690 70 47 51 18 31 33 8 68 46 0 144 180 107 39 40 9 100 100 0 0.152 0.142 0.148 0.145 2.129 0.124 0.546 32 224 121 161 -2.714 16 105 20 141 1629 5764 2.25 15.556 -100 0
3 2540 25670 90 43 44 8 31 33 11 51 11 0 64 64 11 38 40 8 96 78 0 0.462 0.5 0.5 0.45 2.303 0.454 0.305 52 364 38 39 -1.115 12 26 13 26 2277 3324 1 3 0 0
4 2710 25680 100 34 37 12 31 33 25 5 1 0 86 85 13 37 38 8 98 98 0 0.061 0.125 0.116 0.123 2.886 13.798 0.002 80 412 52 54 -4.03 3 49 6 48 2954 6836 0.16 5.875 0 0
5 2890 25680 90 64 65 9 31 32 5 100 100 0 66 68 12 37 38 8 100 84 0 1.138 1.097 1.087 1.144 1.712 1.057 0.439 52 370 62 65 0.186 33 29 34 31 3368 3528 6.6 3.75 0 0
6 3060 25680 90 550 537 87 31 33 16 100 100 0 1328 1283 226 37 41 35 100 100 0 0.402 0.406 0.409 0.408 1.176 0.394 0.95 52 378 1810 1752 -1.315 519 1291 506 1246 27929 66693 31.5 35.486 0 0
7 3250 25680 100 126 119 27 30 32 14 97 92 0 144 138 41 37 40 22 91 87 0 0.897 0.881 0.882 0.955 1.763 0.834 0.88 80 420 203 190 -0.157 96 107 89 101 9550 11012 6.214 4.455 0 0
8 3420 25690 110 86 87 12 29 30 7 100 100 0 692 681 79 37 37 6 100 100 0 0.087 0.09 0.089 0.089 1.264 0.089 0.898 80 429 712 702 -3.522 57 655 58 644 6979 54497 8.143 107.333 0 0
9 3590 25690 100 8748 8479 1918 29 31 10 100 100 0 3119 2932 721 36 37 6 100 100 0 2.828 2.918 2.918 2.953 1.212 2.926 0.969 80 406 11802 11346 1.5 8719 3083 8450 2896 678354 234582 844.8 482.5 0 0
10 3760 25700 110 658 612 153 28 29 4 100 100 0 557 517 126 35 36 6 100 100 0 1.207 1.212 1.185 1.224 1.4 1.221 0.95 80 447 1152 1066 0.271 630 522 584 482 48967 41365 145.75 80.167 0 0
11 3940 25700 110 132 140 37 28 28 4 100 100 0 125 130 38 36 36 6 100 100 0 1.169 1.191 1.216 1.222 1.388 1.166 0.857 80 478 193 206 0.225 104 89 112 94 11209 10399 28 15.667 0 0
12 4120 25710 110 489 499 85 28 29 8 100 100 0 538 551 109 37 38 6 100 100 0 0.92 0.916 0.925 0.921 1.153 0.896 0.932 80 461 962 985 -0.12 461 501 471 514 39885 44043 58.75 85.5 0 0
13 4290 25710 110 122 128 28 27 28 5 100 100 0 138 140 33 37 37 6 100 100 0 0.941 0.981 0.979 0.999 1.354 0.946 0.859 80 450 196 204 -0.088 95 101 101 103 10232 11179 20 17.167 0 0
14 4460 25710 110 37 38 9 28 29 6 62 25 0 66 66 12 35 36 6 96 93 0 0.29 0.323 0.327 0.305 2 0.302 0.167 80 464 40 41 -1.784 9 31 10 31 3015 5240 1.5 5 0 0
15 4640 25710 100 369 338 85 28 29 7 100 100 0 167 161 43 34 35 5 100 100 0 2.564 2.441 2.534 2.542 1.446 2.462 0.918 80 432 474 437 1.358 341 133 310 127 27021 12861 44.143 25.2 0 0
16 4810 25720 90 66 67 11 28 29 5 100 100 0 58 60 13 34 34 6 96 92 0 1.583 1.5 1.464 1.618 1.7 1.431 0.591 52 360 62 65 0.663 38 24 39 26 3507 3103 7.6 4.333 0 0
17 4990 25720 90 46 46 7 28 29 5 96 88 0 50 53 11 34 34 5 92 80 0 1.125 0.947 0.923 0.998 2.039 0.832 0.44 52 354 34 37 0.17 18 16 18 19 2389 2762 3.4 3.8 0 0
18 5150 25690 100 30 33 8 27 28 5 36 20 0 38 40 7 33 34 5 48 28 0 0.6 0.857 0.889 0.802 2.967 0.589 0.063 80 429 8 13 -0.737 3 5 6 7 2611 3206 1 1.2 -50 0
19 5330 25720 100 147 145 29 27 29 6 100 100 0 115 111 27 34 35 6 97 96 0 1.481 1.532 1.637 1.646 1.461 1.517 0.865 80 421 201 195 0.567 120 81 118 77 11581 8875 19.333 12.667 0 0
20 5490 25700 90 52 53 9 28 29 6 100 98 0 55 58 11 34 35 6 98 90 0 1.143 1.042 1.083 1.11 1.676 0.983 0.523 52 356 45 49 0.193 24 21 25 24 2771 3011 4 3.833 0 0

Total number of rows: 5760

Table truncated, full table size 1004 Kbytes.




Supplementary data files not provided

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