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Sample GSM27243 Query DataSets for GSM27243
Status Public on Nov 01, 2005
Title spt5.242.2
Sample type RNA
 
Channel 1
Source name GHY92 (spt5-242) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 57
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R? (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R? (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2900 26110 90 124 127 59 38 39 6 98 94 0 7437 6854 3723 30 33 17 100 100 0 0.012 0.013 0.014 0.019 2.081 0.01 0.568 52 321 7493 6913 -6.428 86 7407 89 6824 6590 356430 14.667 401.235 0 0
2 3090 26120 80 87 104 57 36 37 6 100 94 0 121 175 123 31 31 3 100 100 0 0.567 0.472 0.469 0.476 1.645 0.427 0.738 52 319 141 212 -0.819 51 90 68 144 5424 9080 11.167 48 -100 0
3 3270 26110 90 114 140 86 37 38 6 100 100 0 105 111 28 30 31 3 100 100 0 1.027 1.272 1.053 1.136 1.43 1.703 0.75 52 363 152 184 0.038 77 75 103 81 7281 5755 17 26.667 -100 0
4 3440 26120 100 175 189 64 37 38 6 100 100 0 254 254 25 31 31 4 100 100 0 0.619 0.682 0.614 0.65 1.375 0.708 0.773 80 428 361 375 -0.692 138 223 152 223 15095 20283 25.167 55.75 0 0
5 3620 26120 90 165 182 60 38 38 6 100 100 0 173 173 19 32 32 4 100 100 0 0.901 1.021 0.92 0.964 1.366 1.08 0.817 52 362 268 285 -0.151 127 141 144 141 9440 8994 24 35.25 0 0
6 3800 26130 100 23815 22568 5729 39 43 45 100 100 0 22033 20238 4839 33 33 6 100 100 0 1.081 1.115 1.128 1.115 1.191 1.119 0.983 80 429 45776 42734 0.112 23776 22000 22529 20205 1805441 1619079 500.556 3367.5 0 0
7 3970 26120 100 20607 19930 4269 37 38 6 100 100 0 21366 20157 3941 32 33 4 100 100 0 0.964 0.988 0.973 0.989 1.157 0.99 0.983 80 408 41904 40018 -0.053 20570 21334 19893 20125 1594428 1612534 3315.333 5031 0 0
8 4150 26120 110 2396 2441 395 37 38 6 100 100 0 5216 5356 1010 32 32 4 100 100 0 0.455 0.452 0.452 0.452 1.099 0.443 0.98 80 440 7543 7728 -1.136 2359 5184 2404 5324 195285 428478 400.5 1331 0 0
9 4320 26130 110 12977 11826 3983 37 38 6 100 100 0 9762 9122 2944 32 32 4 100 100 0 1.33 1.297 1.293 1.295 1.241 1.305 0.984 80 455 22670 20879 0.411 12940 9730 11789 9090 946049 729731 1964.667 2272.5 0 0
10 4500 26130 100 1903 1872 334 36 36 5 100 100 0 1327 1328 202 31 31 4 100 100 0 1.441 1.416 1.416 1.414 1.177 1.427 0.972 80 461 3163 3133 0.527 1867 1296 1836 1297 149764 106274 367.2 324.25 0 0
11 4680 26140 110 193 225 120 36 36 6 100 100 0 154 158 32 31 32 4 100 100 0 1.276 1.488 1.318 1.346 1.426 1.804 0.772 80 458 280 316 0.352 157 123 189 127 18006 12660 31.5 31.5 0 0
12 4850 26140 110 334 349 81 35 37 6 100 100 0 298 305 47 31 32 4 100 100 0 1.12 1.146 1.072 1.13 1.256 1.146 0.893 80 417 566 588 0.163 299 267 314 274 27940 24432 52 68.25 0 0
13 5020 26140 110 139 185 153 35 36 5 100 100 0 146 151 33 31 31 4 100 100 0 0.904 1.25 0.939 1.004 1.629 2.014 0.548 80 459 219 270 -0.145 104 115 150 120 14761 12077 29.8 30 -100 0
14 5210 26140 110 95 121 87 36 36 5 100 100 0 121 123 22 30 31 4 100 100 0 0.648 0.914 0.635 0.74 1.667 1.503 0.505 80 472 150 178 -0.625 59 91 85 93 9645 9821 17 23 -100 0
15 5380 26140 100 1574 1480 239 36 36 5 100 100 0 858 813 150 30 31 3 100 100 0 1.857 1.844 1.839 1.854 1.139 1.839 0.978 80 402 2366 2227 0.893 1538 828 1444 783 118391 65056 288.8 260.667 0 0
16 5540 26140 100 190 195 73 35 36 5 100 100 0 137 132 37 30 31 3 100 100 0 1.449 1.569 1.535 1.536 1.337 1.692 0.876 80 414 262 262 0.535 155 107 160 102 15571 10551 31.8 33.667 0 0
17 5710 26120 90 120 130 50 35 36 5 100 100 0 102 101 28 30 30 3 100 100 0 1.181 1.338 1.353 1.297 1.438 1.439 0.79 52 373 157 166 0.239 85 72 95 71 6740 5243 18.8 23.667 0 0
18 5900 26140 100 56 75 56 35 36 5 92 83 0 48 52 15 30 31 3 95 86 0 1.167 1.818 1.216 1.354 2.012 3.277 0.625 80 448 39 62 0.222 21 18 40 22 6032 4160 7.8 7 -100 0
19 6070 26150 100 324 312 86 36 36 6 100 100 0 179 167 44 31 32 4 98 98 0 1.946 2.029 1.968 2.098 1.373 2.053 0.951 80 418 436 412 0.96 288 148 276 136 24926 13337 46 33.75 0 0
20 6240 26120 90 104 136 90 35 36 6 100 100 0 82 88 24 32 32 4 100 100 0 1.38 1.804 1.489 1.547 1.535 2.45 0.746 52 366 119 157 0.465 69 50 101 56 7046 4582 16.667 14 -100 0

Total number of rows: 5760

Table truncated, full table size 1070 Kbytes.




Supplementary data files not provided

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