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Sample GSM27244 Query DataSets for GSM27244
Status Public on Nov 01, 2005
Title spt5.242.3
Sample type RNA
 
Channel 1
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name GHY92 (spt5-242) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
gpr file # 67
 
Submission date Jul 22, 2004
Last update date May 28, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1610 Analyzing the role of transcription elongation factors Spt4-5 in splicing

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R? (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R? (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2430 25490 90 59 61 15 51 53 13 36 15 0 7309 6840 3512 33 36 21 100 100 0 0.001 0.001 0.018 0.018 1.114 0.001 0.06 52 382 7284 6817 -9.829 8 7276 10 6807 3167 355687 0.615 324 0 0
2 2610 25520 60 54 56 10 53 54 13 18 0 0 317 325 201 34 36 9 100 100 0 0.004 0.01 0.033 0.06 3.076 0.005 0.005 32 164 284 294 -8.145 1 283 3 291 1796 10385 0.154 32.111 0 0
3 2770 25520 80 62 64 17 52 54 13 36 11 0 63 66 27 35 36 7 98 82 0 0.357 0.387 0.535 0.468 2.622 0.672 0.252 52 299 38 43 -1.485 10 28 12 31 3336 3408 0.769 4.286 0 0
4 2950 25520 110 191 190 43 52 53 13 98 98 0 132 135 33 35 35 5 100 100 0 1.433 1.38 1.406 1.39 1.35 1.418 0.837 80 454 236 238 0.519 139 97 138 100 15230 10775 10.538 20 0 0
5 3120 25520 90 97 95 21 52 53 15 84 73 0 73 75 14 35 36 9 98 98 0 1.184 1.075 1.105 0.972 2.113 1.389 0.502 52 341 83 83 0.244 45 38 43 40 4923 3910 2.8 4.333 0 0
6 3300 25530 100 6368 6161 2052 54 56 36 100 100 0 5789 5334 1640 35 37 18 100 100 0 1.097 1.152 1.122 1.143 1.216 1.177 0.954 80 408 12068 11406 0.134 6314 5754 6107 5299 492912 426709 169.583 294.278 0 0
7 3470 25520 100 393 372 110 52 53 13 100 100 0 377 362 95 34 35 5 100 100 0 0.994 0.976 0.976 0.958 1.473 1.001 0.941 80 418 684 648 -0.008 341 343 320 328 29784 28958 24.538 65.4 0 0
8 3650 25520 110 1456 1444 204 51 52 12 100 100 0 1138 1118 146 34 35 5 100 100 0 1.273 1.285 1.264 1.281 1.12 1.264 0.962 80 457 2509 2477 0.348 1405 1104 1393 1084 115495 89456 116 216.6 0 0
9 3830 25530 100 3017 2885 878 52 52 13 100 100 0 3079 2943 1076 34 34 5 100 100 0 0.974 0.974 0.977 1.001 1.215 0.949 0.968 80 418 6010 5742 -0.038 2965 3045 2833 2909 230837 235466 217.923 581.8 0 0
10 4000 25540 100 268 279 62 51 53 12 100 100 0 204 201 43 34 35 6 100 100 0 1.276 1.365 1.337 1.373 1.305 1.395 0.904 80 405 387 395 0.352 217 170 228 167 22354 16078 18.833 27.667 0 0
11 4170 25560 90 75 75 15 52 54 14 73 34 0 54 53 8 35 36 8 88 55 0 1.211 1.278 1.081 1.238 2.293 2.321 0.178 52 337 42 41 0.276 23 19 23 18 3876 2777 1.5 2.125 0 0
12 4360 25550 100 165 162 34 52 53 12 100 100 0 124 128 34 35 36 6 100 100 0 1.27 1.183 1.209 1.192 1.528 1.17 0.746 80 404 202 203 0.344 113 89 110 93 12921 10274 9.083 15.333 0 0
13 4540 25560 120 89 88 25 52 54 12 80 69 0 57 57 14 35 36 5 88 74 0 1.682 1.636 1.7 1.736 2.357 2.173 0.452 120 434 59 58 0.75 37 22 36 22 10579 6890 2.833 4.2 0 0
14 4690 25550 100 101 102 22 53 54 12 97 86 0 73 74 12 35 35 5 100 100 0 1.263 1.256 1.25 1.199 1.821 1.643 0.58 80 371 86 88 0.337 48 38 49 39 8180 5909 4 7.8 0 0
15 4860 25560 100 143 146 38 52 53 12 98 98 0 119 118 26 35 35 5 100 98 0 1.083 1.133 1.139 1.091 1.614 1.228 0.718 80 460 175 177 0.115 91 84 94 83 11657 9401 7.75 16.6 0 0
16 5070 25560 60 69 74 27 56 57 14 46 21 0 50 54 22 38 39 8 62 18 0 1.083 1.125 1.274 1.359 3.492 1.372 0.574 32 176 25 34 0.115 13 12 18 16 2355 1715 1.214 1.875 -50 0
17 5230 25540 100 58 58 15 54 55 12 32 10 0 41 42 8 37 37 6 36 18 0 1 0.8 1.9 1.652 3.139 6.635 0.039 80 441 8 9 0 4 4 4 5 4650 3360 0.25 0.833 -50 0
18 5410 25540 100 57 57 13 54 55 12 22 5 0 39 40 6 36 36 5 35 13 0 1 0.75 3.333 2.759 2.878 14.634 0.017 80 457 6 7 0 3 3 3 4 4540 3186 0.167 0.8 -50 0
19 5610 25570 100 180 182 38 53 55 12 100 100 0 176 170 38 36 37 5 100 100 0 0.907 0.963 0.959 0.972 1.466 0.966 0.831 80 463 267 263 -0.141 127 140 129 134 14579 13596 10.583 26.6 0 0
20 5750 25570 70 73 73 13 54 55 13 71 21 0 57 57 9 37 37 5 96 87 0 0.95 0.95 1 0.955 2.4 1.674 0.225 32 216 39 39 -0.074 19 20 19 20 2349 1829 1.385 4 0 0

Total number of rows: 5760

Table truncated, full table size 997 Kbytes.




Supplementary data files not provided

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