|
| Status |
Public on Jun 01, 2018 |
| Title |
Venous blood_d34_ctrl_4641 |
| Sample type |
RNA |
| |
|
| Source name |
female_control_whole blood
|
| Organism |
Gallus gallus |
| Characteristics |
chicken id: chicken 4641
gender: female
age: 34 days after birth
sample group: control
tissue: whole, venous blood
|
| Treatment protocol |
For 24h after leaving the hatchery, the chicks were kept without food and water in containers and subjected to a lower room temperature (22°C = cold vs 27°C = control) and shaking movements mimicking transport. Control animals were not be subjected to stressors after leaving the hatchery and were placed in standard rearing conditions. Venous blood was collected from the 24 chicks 34 days after laying, using vacuum tubes with EDTA. 100µl of whole blood were diluted in 1ml of trizol. After a strong shaking and 5 mn of incubation at RT, the tubes were frozen in liquid nitrogen and stored at -80°C until extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Before extraction, 10µl of acetic acid 5N was added in each tube. Total RNA was extracted with TRIzol reagent (Invitrogen) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions, followed by Agencourt RNAClean XP (Agencourt Bioscience Corporation, Beverly, Massachusetts). Dye incorporation and cRNA yield were checked using Dropsense™ 96 UV/VIS droplet reader (Trinean, Belgium).
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labelled cRNA were hybridized on the microarray slides following the manufacturer’s instructions.
|
| Scan protocol |
Immediately after washing, the slides were scanned on Agilent G2505C Microarray Scanner using Agilent Scan Control A.8.5.1 software.
|
| Description |
SAMPLE 12
|
| Data processing |
The fluorescence signal extracted using Agilent Feature Extraction software v10.10.1.1 with default parameters.
Raw data (median signal intensity) were filtered, log2 transformed, and normalized using quantile method.
|
| |
|
| Submission date |
Aug 08, 2017 |
| Last update date |
Jun 01, 2018 |
| Contact name |
Aline FOURY |
| E-mail(s) |
[email protected]
|
| Organization name |
INRAE
|
| Lab |
NutriNeuro
|
| Street address |
146 rue Léo Saignat
|
| City |
Bordeaux |
| ZIP/Postal code |
33600 |
| Country |
France |
| |
|
| Platform ID |
GPL19630 |
| Series (1) |
| GSE102358 |
Spontaneous intake of essential oils after a negative postnatal experience has long-term effects on blood transcriptome in chickens |
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