|
| Status |
Public on May 08, 2018 |
| Title |
Spleen_GR-C3 knockin_LPS_3hr_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
spleen, GR-C3 knockin, LPS-treated, 3hour
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
genetic background: C57BL/6
gender: GR-C3 knockin
agent: LPS
time: 3hour
tissue: spleen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from WT and rescued GR-C3 knockin spleens using TRIzol reagent according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng of each total RNA sample was labeled according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was done according to the manufacturer's instructions.
|
| Scan protocol |
Gene expression was quantified on the nCounter Digital AnalyzerTM.
|
| Description |
C3-LPS-3
|
| Data processing |
Data were normalized utilizing the manufacturer’s positive and negative experimental control probes, as well as all 4 housekeeping genes (G6pdx, Ppia, Oaz1, and Rpl19)
|
| |
|
| Submission date |
Sep 11, 2017 |
| Last update date |
May 08, 2018 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL19964 |
| Series (1) |
| GSE103721 |
Inflammatory Gene Changes in Spleens from WT and Rescued GR-C3 Knockin Mice Treated with LPS |
|
