|
| Status |
Public on Mar 01, 2009 |
| Title |
Tibialis anterior muscle from MLC-SOCS-3/C57Bl6J mice rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Tibialis anterior muscle
|
| Organism |
Mus musculus |
| Characteristics |
C57Bl6J mice
transgenic animals expressing constitutively SOCS-3 specifically in skeletal muscle (under the control of MLC-1 promoter)
|
| Treatment protocol |
Tibialis muscle was extracted from 6h starved mice and quickly frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 3ug of DNA were hybridized for 17 hr at 45C on Mouse gene 1.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from tibialis muscle extracted from 11 week-old mouse
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings and Gene Level-RMA sketch as normalization method.
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| |
|
| Submission date |
Apr 09, 2008 |
| Last update date |
Nov 21, 2008 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE11105 |
Expression data from tibialis anterior muscle |
|
