|
| Status |
Public on Oct 19, 2017 |
| Title |
vessel at T3, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse vessel Angiotensin II treated for 3days
|
| Organism |
Mus musculus |
| Characteristics |
tissue: vessel
agent: Angiotensin II
time point: 3 days
stain: C57BL/6
|
| Treatment protocol |
Mice were implanted with osmotic pumps (Alzet Model 1007D; Cupertino, CA,USA) and infused with Angiotensin II at 1500ng/kg/min in Ringer's solution (0.01mmol/L acetic acid in saline) for 1, 3, and 7 days . Systolic blood pressure was measured by the tail-cuff method before starting treatment and again every day after Angiotensin II infusion.
|
| Growth protocol |
Eight to ten-week-old C57BL/6 male mice were kept at least 1 wk at 22°C and 55% relative humidity in a 12-h day/night lighting environment with free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All mice were euthanized by an overdose of pentobarbital on days 1, 3 and 7 of Ang II treatment. Total RNA was isolated with TRIzol (Invitrogen) from vessels at each time point according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Affymetrix Mouse Transcriptome Array 1.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Genechips were scanned by GeneChip® Scanner 3000.
|
| Description |
Gene expression data from mouse vessel Angiotensin II treated for 3days
|
| Data processing |
The data were analyzed with Expression Console™, Version 1.3.1.
|
| |
|
| Submission date |
Oct 18, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE105114 |
Expression data from mouse tissue Angiotensin II treated for 0, 1, 3, 7 days |
|
