|
| Status |
Public on Jan 11, 2018 |
| Title |
Fertile cow_003 |
| Sample type |
RNA |
| |
|
| Source name |
Endometrial tissue in fertile cow_003
|
| Organism |
Bos taurus |
| Characteristics |
animal no.: 425
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the endometrial samples using RNeasy Plus Universal Mini kit (Qiagen) according to the manufacturer’s instructions. Total RNA quality and quantity were confirmed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and a NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA), respectively.
|
| Label |
Cy3
|
| Label protocol |
One-color spike-mix was added to the total RNA prior to the labeling reaction. Labeling was performed using a Quick Amp Labeling Kit, One-Color (Agilent Technologies) in the presence of cyanine-3 (Cy3)-CTP according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
For microarray hybridization, 1650 ng of Cy3-labeled cRNA was fragmented and hybridized on a slide of bovine 4 × 44K microarray (Agilent Technologies; 023647) at 65°C for 17 hours.
|
| Scan protocol |
Slides were scanned on the Agilent G2505C DNA microarray scanner.
|
| Data processing |
Background correction of the Cy3 raw signals was performed using the Agilent Feature Extraction software (version 10.5.1.1). Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Dec 06, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Shuichi Matsuyama |
| E-mail(s) |
[email protected]
|
| Organization name |
Nagoya University
|
| Lab |
Animal Production Science
|
| Street address |
94 Hatajiri, Morowa
|
| City |
Aichi-gun |
| ZIP/Postal code |
470-0151 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11649 |
| Series (1) |
| GSE107741 |
Endometrial gene expression in fertile and subfertile cows |
|
