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Sample GSM28994 Query DataSets for GSM28994
Status Public on Jun 13, 2005
Title wt_SC5_3
Sample type RNA
 
Channel 1
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name OY94 (wt) @ 39 deg
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description Reference and mutant strains grown at 30oC to 1-2 x 10exp7 cells/ml. shifted to 39 deg for 45 minutes. RNA extracted.
 
Submission date Aug 12, 2004
Last update date Jun 13, 2005
Contact name Todd A Burckin
E-mail(s) [email protected]
Phone 831 459-5245
Fax 831 459-3139
Organization name University of California Santa Cruz
Department Molecular, Cell, & Developmental Biology
Lab Hartzog Lab
Street address 1156 High Street
City Santa Cruz
State/province CA
ZIP/Postal code 95062
Country USA
 
Platform ID GPL1370
Series (1)
GSE1684 Exploring functional relationships between components of the gene expression machinery

Data table header descriptions
ID_REF
X X-coordinate of spot
Y Y-coordinate of spot
Dia. spot diameter in microns
F635 Median Channel 2 (Cy5/635) median pixel intensity - feature
F635 Mean Channel 2 (Cy5/635) mean pixel intensity - feature
F635 SD Channel 2 (Cy5/635) standard deviation of pixel intensities - feature
B635 Median Channel 2 (Cy5/635) median background intensity
B635 Mean Channel 2 (Cy5/635) mean background intensity
B635 SD Channel 2 (Cy5/635) standard deviation of background
% > B635+1SD Channel 2 (Cy5/635) percent of pixels 1 StDev above background
% > B635+2SD Channel 2 (Cy5/635) percent of pixels 2 StDevs above background
F635 % Sat. Channel 2 (Cy5/635) percent of pixels saturated
F532 Median Channel 1 (Cy3/532) median pixel intensity - feature
F532 Mean Channel 1 (Cy3/532) mean pixel intensity - feature
F532 SD Channel 1 (Cy3/532) standard deviation of pixel intensities - feature
B532 Median Channel 1 (Cy3/532) median background intensity
B532 Mean Channel 1 (Cy3/532) mean background intensity
B532 SD Channel 1 (Cy3/532) standard deviation of background
% > B532+1SD Channel 1 (Cy3/532) percent of pixels 1 StDev above background
% > B532+2SD Channel 1 (Cy3/532) percent of pixels 2 StDevs above background
F532 % Sat. Channel 1 (Cy3/532) percent of pixels saturated
Ratio of Medians (635/532)
Ratio of Means (635/532)
Median of Ratios (635/532)
Mean of Ratios (635/532)
Ratios SD (635/532)
Rgn Ratio (635/532)
Rgn R� (635/532)
F Pixels Number of feature pixels
B Pixels Number of background pixels
Sum of Medians Sum of medians
Sum of Means Sum of means
VALUE Log ratio (base 2)
F635 Median - B635 Channel 2 (Cy5/635) background subtracted median feature intensity
F532 Median - B532 Channel 1 (Cy3/532) background subtracted median feature intensity
F635 Mean - B635 Channel 2 (Cy5/635) background subtracted mean feature intensity
F532 Mean - B532 Channel 1 (Cy3/532) background subtracted mean feature intensity
F635 Total Intensity F635 Total Intensity
F532 Total Intensity F532 Total Intensity
SNR 635 n/a
SNR 532 n/a
Flags User defined flag (default=0)
Normalize n/a

Data table
ID_REF X Y Dia. F635 Median F635 Mean F635 SD B635 Median B635 Mean B635 SD % > B635+1SD % > B635+2SD F635 % Sat. F532 Median F532 Mean F532 SD B532 Median B532 Mean B532 SD % > B532+1SD % > B532+2SD F532 % Sat. Ratio of Medians (635/532) Ratio of Means (635/532) Median of Ratios (635/532) Mean of Ratios (635/532) Ratios SD (635/532) Rgn Ratio (635/532) Rgn R� (635/532) F Pixels B Pixels Sum of Medians Sum of Means VALUE F635 Median - B635 F532 Median - B532 F635 Mean - B635 F532 Mean - B532 F635 Total Intensity F532 Total Intensity SNR 635 SNR 532 Flags Normalize
1 2800 26060 100 80 81 27 33 35 11 91 80 0 12607 12908 8375 34 38 14 100 100 0 0.004 0.004 0.021 0.024 2.608 0.003 0.812 80 384 12620 12922 -8.063 47 12573 48 12874 6506 1032601 4.182 919.286 0 0
2 2970 26060 100 49 58 26 32 33 6 80 60 0 85 156 136 34 36 8 93 90 0 0.333 0.213 0.22 0.258 2.51 0.186 0.615 80 415 68 148 -1.585 17 51 26 122 4653 12441 4.167 15 -100 0
3 3150 26060 90 68 68 10 32 32 5 100 100 0 109 106 18 33 34 6 100 100 0 0.474 0.493 0.495 0.489 1.458 0.479 0.767 52 360 112 109 -1.078 36 76 36 73 3516 5501 7.2 12 0 0
4 3330 26060 100 52 59 24 32 34 8 88 58 0 53 64 26 34 36 9 81 51 0 1.053 0.9 0.906 0.931 2.041 0.918 0.726 80 415 39 57 0.074 20 19 27 30 4742 5097 3.125 3.111 -100 0
5 3500 26070 100 97 96 25 33 34 7 98 96 0 111 109 26 34 36 9 98 97 0 0.831 0.84 0.85 0.819 1.507 0.837 0.831 80 406 141 138 -0.267 64 77 63 75 7652 8715 8.857 8.111 0 0
6 3670 26070 100 1100 1019 270 33 34 7 100 100 0 1431 1322 343 34 37 14 100 100 0 0.764 0.766 0.77 0.767 1.332 0.764 0.976 80 419 2464 2274 -0.389 1067 1397 986 1288 81552 105761 140.714 91.786 0 0
7 3850 26060 110 274 263 65 33 35 15 100 98 0 329 315 73 34 39 22 100 100 0 0.817 0.819 0.804 0.799 1.222 0.817 0.951 80 464 536 511 -0.292 241 295 230 281 21052 25231 15.2 12.545 0 0
8 4030 26070 110 126 127 20 33 34 7 100 100 0 152 154 28 34 36 10 100 100 0 0.788 0.783 0.78 0.791 1.271 0.773 0.896 80 459 211 214 -0.343 93 118 94 120 10183 12314 13.286 11.8 0 0
9 4200 26080 100 11274 10566 2869 33 34 8 100 100 0 13394 12127 3752 34 35 8 100 100 0 0.841 0.871 0.866 0.902 1.254 0.855 0.981 80 406 24601 22626 -0.249 11241 13360 10533 12093 845289 970126 1316.5 1511.5 0 0
10 4370 26080 100 1547 1465 254 33 34 10 100 100 0 1554 1474 263 33 37 16 100 100 0 0.995 0.994 0.995 0.995 1.104 0.826 0.835 80 405 3035 2873 -0.007 1514 1521 1432 1441 117235 117923 143.1 89.813 0 0
11 4550 26090 110 72 81 29 33 33 6 100 100 0 79 85 32 34 35 6 98 97 0 0.867 0.941 0.891 0.933 1.535 0.883 0.745 80 461 84 99 -0.206 39 45 48 51 6472 6815 8 8.333 0 0
12 4720 26080 100 163 170 50 32 33 7 100 100 0 192 195 37 34 35 7 100 100 0 0.829 0.857 0.857 0.847 1.214 0.901 0.896 80 432 289 299 -0.27 131 158 138 161 13622 15582 19.571 22.857 0 0
13 4900 26090 110 73 81 42 32 33 6 100 97 0 78 99 95 34 36 9 97 95 0 0.932 0.754 0.832 0.874 1.627 0.502 0.757 80 465 85 114 -0.102 41 44 49 65 6469 7882 8 7 -100 0
14 5070 26100 100 58 64 28 32 33 6 93 87 0 62 80 47 33 35 9 96 77 0 0.897 0.681 0.742 0.755 1.927 0.614 0.769 80 404 55 79 -0.158 26 29 32 47 5123 6391 5.167 5 -100 0
15 5240 26100 100 1024 946 250 33 34 8 100 100 0 1146 1035 288 33 35 11 100 100 0 0.89 0.911 0.925 0.927 1.258 0.905 0.974 80 400 2104 1915 -0.167 991 1113 913 1002 75712 82776 114 90.909 0 0
16 5410 26090 100 121 112 30 32 35 10 98 96 0 127 123 34 34 37 16 97 93 0 0.957 0.899 0.927 0.91 1.377 0.894 0.879 80 432 182 169 -0.063 89 93 80 89 8973 9800 7.7 5.375 0 0
17 5580 26100 90 74 83 26 33 35 10 100 96 0 81 89 23 33 37 13 100 98 0 0.854 0.893 0.863 0.863 1.451 0.943 0.767 52 331 89 106 -0.227 41 48 50 56 4309 4624 4.8 4 0 0
18 5750 26090 120 45 50 21 34 38 19 32 9 0 49 52 21 34 41 27 26 5 0 0.733 0.889 1 0.946 2.329 0.7 0.579 120 537 26 34 -0.447 11 15 16 18 6001 6272 0.632 0.407 -50 0
19 5930 26100 90 223 221 31 33 36 12 100 100 0 226 223 30 33 38 20 100 100 0 0.984 0.989 1.02 0.984 1.178 0.996 0.932 52 352 383 378 -0.023 190 193 188 190 11481 11611 15.417 9.25 0 0
20 6100 26080 90 67 69 13 33 44 104 0 0 0 76 81 18 33 40 48 38 3 0 0.791 0.75 0.765 0.744 1.523 0.744 0.737 52 372 77 84 -0.339 34 43 36 48 3575 4204 0.24 0.854 -50 0

Total number of rows: 5760

Table truncated, full table size 1045 Kbytes.




Supplementary data files not provided

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