Total RNA was extracted with High Pure RNA Paraffin Kit (Roche)
Label
SYBR Green
Label protocol
First-strand cDNA was synthesized using Superscript III reverse transcriptase (first Strand Kit; Invitrogen, Carlsbad, CA) and a mixture of random hexamers and gene-specific primers. PCR amplification and fluorescent melting curve analysis was done on the LightCycler 480 using SYBR Green I Master Mix (Roche Applied Science).
Hybridization protocol
n/a
Scan protocol
n/a
Description
breast tumor
Data processing
Each gene was normalized to the expression of the same gene in a within-run reference sample and then normalized to the geometric average of the 5 housekeeper genes (ACTB, MRPL19, PSMC4, RPLP0, SF3A1)
