|
| Status |
Public on Feb 22, 2019 |
| Title |
Phosphate ZnO-MNP - P1 |
| Sample type |
RNA |
| |
|
| Source name |
C. elegans exposed to phosphate aged ZnO-MNPs
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
age: started at L7
treatment: phosphate aged ZnO-MNPs
|
| Treatment protocol |
Exposed to ZnO-MNPs in pristine and aged forms as wel ZnSO4 for 72 hr
|
| Growth protocol |
L1 were placed into Simulated soil pore water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol with Qiagen RNeasy Kit
|
| Label |
biotin
|
| Label protocol |
A total of 100 ng of total RNA was used to generate fragmented, labeled ss-cDNA using Affymetrix WT Plus kit.
|
| |
|
| Hybridization protocol |
5.0 µg of the fragmented, labeled ss-cDNA were hybridized to EleGene 1.0 ST arrays for 16 h at 45°C and shaken at 60 RPM.
|
| Scan protocol |
Affymetrix GeneChip 7G scanner
|
| Data processing |
Raw data files were normalized using the robust multi-array average (RMA) algorithm followed by quantile normalization implemented in Partek Genomics Suite 6.4
|
| |
|
| Submission date |
May 24, 2018 |
| Last update date |
Feb 22, 2019 |
| Contact name |
Olga Tsyusko |
| E-mail(s) |
[email protected]
|
| Phone |
859-257-1777
|
| Organization name |
University of Kentucky
|
| Department |
Plant and Soil Sciences
|
| Street address |
1100 S. Limestone St
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40546 |
| Country |
USA |
| |
|
| Platform ID |
GPL19230 |
| Series (1) |
| GSE114881 |
Expression data from C. elegans exposed to pristine and aged ZnO manufactured nanoparticles |
|
