|
| Status |
Public on Sep 10, 2018 |
| Title |
WT_AJ_1 |
| Sample type |
RNA |
| |
|
| Source name |
Lung
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Two weeks Dox induction
strain: AJ
genotype: WT
fibrosis: 1, prone; 2, resistant strains: 2
emphysematous: NULL
|
| Treatment protocol |
All the mice were given 2weeks of Doxycycline in a drinking water for induction of TGF-b transgene expression
|
| Growth protocol |
Freshly harvested lungs in PBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fresh lung tissue was homogenized in a Trisol solution and followed by RNA purificaton using Qiagen RNA purification kit
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression Kit was used for cDNA synthesis and IVT labeling
|
| |
|
| Hybridization protocol |
GeneChip Scanner 3000
|
| Scan protocol |
A “rotisserie” oven is used to carry out the hybridization of up to 64 arrays at one time.
|
| Description |
lung, Rt lobe
|
| Data processing |
Affy Package (1.58.0) in R (3.3.2) was used to processing of raw data, no filtering was performed.
|
| |
|
| Submission date |
Sep 07, 2018 |
| Last update date |
Sep 10, 2018 |
| Contact name |
Chun Geun Lee |
| E-mail(s) |
[email protected]
|
| Phone |
401-863-5932
|
| Organization name |
Brown University
|
| Department |
Molecular Microbiology and Immunology
|
| Street address |
185 Meeting St
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE119662 |
Genetic modifier of TGF-beta1 stimulated pulmonayr fibrosis |
|
