|
| Status |
Public on Apr 11, 2010 |
| Title |
PBMC 25 PUFA 6 h after intake |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells, PUFA shake, 6 hours after intake, individual 25
|
| Organism |
Homo sapiens |
| Characteristics |
Male, 18-27 years of age
|
| Treatment protocol |
Subjects were given a shake containing either SFA or PUFA as the fatty acid source as a single challange consumption
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were lysed in Qiagen RLT buffer and homogenized using seringes with 0.4 mm needles, whereafter purified total RNA was isolated using Qiagen RNEasy columns
|
| Label |
biotin
|
| Label protocol |
TThe Ambion MessageAmp aRNA Amplification Kit was used to prepare labelled cRNA from 500ng of total RNA. The protocol was conducted using the reagents provided by Ambion (P/N 1751), as per the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridisation of 10ug cRNA was done overnight for 16 hours at 45ºC in a Hybridisation Oven 640 (Affymetrix). The protocol is conducted as described in the "Genechip Expression Analysis Technical Manual", section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Target Hybridization) (P/N 701027, revision 5).
|
| Scan protocol |
Arrays were scanned on an Affymetrix 3000 7G scanner, as described n the "Genechip Expression Analysis Technical Manual", section 2 (Eukaryotic Sample and Array Processing), chapter 2 (Eukaryotic Arrays: Washing, Staining and Scanning (P/N 701028, revision 5).
|
| Description |
none
|
| Data processing |
Expression estimates were calculated using RMA
|
| |
|
| Submission date |
Nov 04, 2008 |
| Last update date |
Apr 11, 2010 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL7144 |
| Series (1) |
| GSE13466 |
Acute effects of different dietary fatty acids on the gene expression profiles of PBMCs of healthy young men |
|
