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Sample GSM34696 Query DataSets for GSM34696
Status Public on Nov 06, 2004
Title YSS89, T=60, b
Sample type RNA
 
Source name Yeast
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Description Experiment Design:
•Type of experiment: time course after GAL (galactose) induction
•Experimental factor: time, genotype
•Number of hybridizations performed in the experiment: 24 (eight conditions with three biological replicates each)


Samples used, extract preparation and labeling:
•The origin of the biological sample:
Species: S. cerevisiae
Cell type:
wild-type (YSS37, IFH1-13myc-TRP1, MATa)
UASIFH1::HIS3-UASGAL1 (YSS89, UASGAL1-IFH1-13myc-TRP1, MATa)
•Growth conditions: log phase (2x107/mL) at 30°C in YPLG medium, then addition of Galactose to 2%
•Treatments: all cells were harvested by rapid centrifugation at 4°C then flash frozen in liquid nitrogen.
•RNA extraction: RNeasy Protect mini kit (Quiagen)
•Labeling protocol(s):
cDNA synthesis using Invitrogen reagents according to Affymetrix protocol (5 µg of total RNA)
GeneChip IVT labeling kit (Affymetrix)
•External controls (spikes): according to the Affymetrix protocols
Hybridization controls
Poly-A spike controls
Keywords = IFH1
Keywords = Transcriptional control of RP genes
 
Submission date Nov 05, 2004
Last update date Sep 08, 2006
Contact name Stephan B. Schawalder
E-mail(s) [email protected]
Phone +41 22 379 61 77
Organization name University of Geneva
Department Departement of Molecular biology
Lab Group Shore
Street address Quai Ansermet 30
City Geneva
ZIP/Postal code 1211
Country Switzerland
 
Platform ID GPL90
Series (1)
GSE1934 Ifh1 activates RP genes

Data table header descriptions
ID_REF
VALUE Expression measure signal
ABS_CALL Detection flag (present, marginal or absent)
DETECTION P-VALUE Detection flag p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 1 A 0.9273
AFFX-MurIL10_at 0.3 A 0.852061
AFFX-MurIL4_at 0.3 A 0.981719
AFFX-MurFAS_at 0.6 A 0.883887
AFFX-BioB-5_at 52.1 P 0.000446
AFFX-BioB-M_at 68.7 P 0.000052
AFFX-BioB-3_at 53.2 P 0.000258
AFFX-BioC-5_at 156.7 P 0.000052
AFFX-BioC-3_at 170.2 P 0.000044
AFFX-BioDn-5_at 314.5 P 0.000044
AFFX-BioDn-3_at 1019.8 P 0.000044
AFFX-CreX-5_at 1795.9 P 0.000044
AFFX-CreX-3_at 2063.2 P 0.000044
AFFX-BioB-5_st 1 A 0.737173
AFFX-BioB-M_st 0.7 A 0.843268
AFFX-BioB-3_st 0.7 A 0.916408
AFFX-BioC-5_st 3.8 A 0.645547
AFFX-BioC-3_st 1 A 0.58862
AFFX-BioDn-5_st 1.5 A 0.645547
AFFX-BioDn-3_st 16 P 0.033677

Total number of rows: 9335

Table truncated, full table size 220 Kbytes.




Supplementary file Size Download File type/resource
GSM34696.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM34696.EXP.gz 346 b (ftp)(http) EXP
GSM34696.RPT.gz 903 b (ftp)(http) RPT

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